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Literature summary extracted from

  • Wheeler, P.R.; Bharadwaj, V.P.; Gregory, D.
    N-Acetyl-beta-glucosaminidase, beta-glucuronidase and acid phosphatase in Mycobacterium leprae (1982), J. Gen. Microbiol., 128, 1063-1071.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.52 acetate untreated, concentration of 50 mM: activity reaches 170% Mycobacterium leprae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 7-diazonaphthalene-1,3-disulfonic acid extract: 45% of activity remains Mycobacterium leprae
3.2.1.52 acetate with 50 mM 45% of activity remains Armadillo officinalis
3.2.1.52 N-acetyl-beta-D-glucosaminolactone with 0.02 30% of activity remains Armadillo officinalis
3.2.1.52 N-acetyl-beta-D-glucosaminolactone untreated: with 0.02 mM 65% of activity remains, treated with 7-diazonaphtalene-1,3-disulfonic acid: with 0.02 mM 70% of activity remains Mycobacterium leprae
3.2.1.52 NaOH unbroken bacteria: total inhibition with 1 M, extract: with 1 M 9% of activity remains Mycobacterium leprae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.52 membrane
-
 Mycolicibacterium phlei 16020
-
3.2.1.52 membrane
-
 Mycobacterium tuberculosis variant microti 16020
-
3.2.1.52 membrane
-
 Mycobacterium leprae 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Mycolicibacterium phlei
-
 N-acetyl-D-hexosamines
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Mycobacterium tuberculosis variant microti
-
 N-acetyl-D-hexosamines
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Mycobacterium leprae
-
 N-acetyl-D-hexosamines
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Armadillo officinalis
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.31 Mycobacterium leprae
-
-
-
3.2.1.52 Armadillo officinalis
-
-
-
3.2.1.52 Mycobacterium leprae
-
 from armadillo liver
-
3.2.1.52 Mycobacterium tuberculosis variant microti
-
-
-
3.2.1.52 Mycolicibacterium phlei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 30fold Mycobacterium leprae
3.2.1.52 30fold Armadillo officinalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.31 additional information from armadillo liver infected with Mycobacterium leprae Mycobacterium leprae
-
3.2.1.52 liver
-
 Armadillo officinalis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 additional information
-
-
 Mycolicibacterium phlei
3.2.1.52 additional information
-
-
 Mycobacterium tuberculosis variant microti
3.2.1.52 additional information
-
-
 Mycobacterium leprae
3.2.1.52 additional information
-
-
 Armadillo officinalis

Storage Stability

EC Number Storage Stability Organism
3.2.1.52 -80°C, crude cell-free extract, 0.25-2.0 mg protein/ml Mycolicibacterium phlei
3.2.1.52 -80°C, crude cell-free extract, 0.25-2.0 mg protein/ml Mycobacterium tuberculosis variant microti
3.2.1.52 -80°C, crude cell-free extract, 0.25-2.0 mg protein/ml Mycobacterium leprae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Mycolicibacterium phlei N-acetyl-D-hexosamines
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Mycobacterium tuberculosis variant microti N-acetyl-D-hexosamines
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Mycobacterium leprae N-acetyl-D-hexosamines
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Armadillo officinalis N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Mycolicibacterium phlei 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Mycobacterium tuberculosis variant microti 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Mycobacterium leprae 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Armadillo officinalis 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 8.5 9 inactivation at high pH like 8.5-9.0 Armadillo officinalis