BRENDA - Enzyme Database

Purification and some properties of hydroxypyruvate isomerase of Bacillus fastidiosus

De Windt, F.E.; van der Drift, C.; Biochim. Biophys. Acta 613, 556-562 (1980)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
5.3.1.22
loss of activity by vacuum dialysis at 4°C or by freezing
Bacillus fastidiosus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.1.22
Cu2+
0.1 mM, 57% inhibition
Bacillus fastidiosus
5.3.1.22
Fe2+
0.1 mM, 18% inhibition
Bacillus fastidiosus
5.3.1.22
hydroxylamine
20 mM, 60% inhibition
Bacillus fastidiosus
5.3.1.22
iodoacetate
0.7 mM, 100% inhibition
Bacillus fastidiosus
5.3.1.22
NaCN
2.5 mM, 21% inhibition
Bacillus fastidiosus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.1.22
10
-
Hydroxypyruvate
-
Bacillus fastidiosus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.1.22
45000
-
6 * 45000, SDS-PAGE
Bacillus fastidiosus
5.3.1.22
265000
-
gel filtration
Bacillus fastidiosus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.1.22
Bacillus fastidiosus
-
strain DSM 83
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.1.22
-
Bacillus fastidiosus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.3.1.22
3125
-
-
Bacillus fastidiosus
Storage Stability
EC Number
Storage Stability
Organism
5.3.1.22
4°C, stable for 1 month
Bacillus fastidiosus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.1.22
Hydroxypyruvate
r
2658
Bacillus fastidiosus
2-Hydroxy-3-oxopropanoate
r
2658
Bacillus fastidiosus
-
Subunits
EC Number
Subunits
Commentary
Organism
5.3.1.22
hexamer
6 * 45000, SDS-PAGE
Bacillus fastidiosus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
5.3.1.22
57
-
-
Bacillus fastidiosus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
5.3.1.22
67
-
10 min stable
Bacillus fastidiosus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3.1.22
6.6
7.4
-
Bacillus fastidiosus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
5.3.1.22
5.4
8.5
50% of maximal activity at pH 5.4 and 8.5
Bacillus fastidiosus
General Stability (protein specific)
EC Number
General Stability
Organism
5.3.1.22
loss of activity by vacuum dialysis at 4°C or by freezing
Bacillus fastidiosus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.1.22
Cu2+
0.1 mM, 57% inhibition
Bacillus fastidiosus
5.3.1.22
Fe2+
0.1 mM, 18% inhibition
Bacillus fastidiosus
5.3.1.22
hydroxylamine
20 mM, 60% inhibition
Bacillus fastidiosus
5.3.1.22
iodoacetate
0.7 mM, 100% inhibition
Bacillus fastidiosus
5.3.1.22
NaCN
2.5 mM, 21% inhibition
Bacillus fastidiosus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.1.22
10
-
Hydroxypyruvate
-
Bacillus fastidiosus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.1.22
45000
-
6 * 45000, SDS-PAGE
Bacillus fastidiosus
5.3.1.22
265000
-
gel filtration
Bacillus fastidiosus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.1.22
-
Bacillus fastidiosus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.3.1.22
3125
-
-
Bacillus fastidiosus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
5.3.1.22
4°C, stable for 1 month
Bacillus fastidiosus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.1.22
Hydroxypyruvate
r
2658
Bacillus fastidiosus
2-Hydroxy-3-oxopropanoate
r
2658
Bacillus fastidiosus
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.3.1.22
hexamer
6 * 45000, SDS-PAGE
Bacillus fastidiosus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
5.3.1.22
57
-
-
Bacillus fastidiosus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
5.3.1.22
67
-
10 min stable
Bacillus fastidiosus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3.1.22
6.6
7.4
-
Bacillus fastidiosus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
5.3.1.22
5.4
8.5
50% of maximal activity at pH 5.4 and 8.5
Bacillus fastidiosus