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Literature summary extracted from

  • Nakada, T.; Maruta, K.; Mitsuzumi, H.; Kubota, M.; Chaen, H.; Sugimoto, T.; Kurimoto, M.; Tsujisaka, Y.
    Purification and characterization of a novel enzyme, maltooligosyl trehalose trehalohydrolase, from Arthrobacter sp. Q36 (1995), Biosci. Biotechnol. Biochem., 59, 2215-2218.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.141 Cu2+
-
 Arthrobacter sp.
3.2.1.141 Fe2+
-
 Arthrobacter sp.
3.2.1.141 Hg2+
-
 Arthrobacter sp.
3.2.1.141 Zn2+
-
 Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.141 4.2
-
 maltopentaosyl trehalose
-
 Arthrobacter sp.
3.2.1.141 4.6
-
 maltotriosyl trehalose
-
 Arthrobacter sp.
3.2.1.141 5.5
-
 maltosyl trehalose
-
 Arthrobacter sp.
3.2.1.141 7
-
 maltotetraosyl trehalose
-
 Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.141 Arthrobacter sp.
-
-
-
3.2.1.141 Arthrobacter sp. O36
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.141
-
 Arthrobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.141 497
-
-
 Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.141 maltopentaosyltrehalose + H2O
-
 Arthrobacter sp. maltopentaose + alpha,alpha'-trehalose
-
 ?
3.2.1.141 maltopentaosyltrehalose + H2O
-
 Arthrobacter sp. O36 maltopentaose + alpha,alpha'-trehalose
-
 ?
3.2.1.141 maltosyltrehalose + H2O
-
 Arthrobacter sp. maltose + alpha,alpha'-trehalose
-
 ?
3.2.1.141 maltosyltrehalose + H2O
-
 Arthrobacter sp. O36 maltose + alpha,alpha'-trehalose
-
 ?
3.2.1.141 maltotetraosyltrehalose + H2O
-
 Arthrobacter sp. maltotetraose + alpha,alpha'-trehalose
-
 ?
3.2.1.141 maltotetraosyltrehalose + H2O
-
 Arthrobacter sp. O36 maltotetraose + alpha,alpha'-trehalose
-
 ?
3.2.1.141 maltotriosyltrehalose + H2O
-
 Arthrobacter sp. maltotriose + alpha,alpha'-trehalose
-
 ?
3.2.1.141 maltotriosyltrehalose + H2O
-
 Arthrobacter sp. O36 maltotriose + alpha,alpha'-trehalose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.141 45
-
-
 Arthrobacter sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.141 30 55 30°C: about 75% of maximal activity, 55°C: about 40% of maximal activity Arthrobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.141 45
-
 60 min, stable up to Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.141 6.5
-
-
 Arthrobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.141 5 8 pH 5: about 50% of maximal activity, pH 8: about 40% of maximal activity Arthrobacter sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.141 5 10 4°C, 24 h, stable Arthrobacter sp.