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Literature summary extracted from

  • Sano, M.; Hayakawa, K.; Kato, I.
    Purification and characterization of an enzyme releasing lacto-N-biose from oligosaccharides with type 1 chain (1993), J. Biol. Chem., 268, 18560-18566.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.140 dilution without Brij58 causes 30-50% inactivation Streptomyces sp.
3.2.1.140 stabilized by 0.05% Brij58, 0.05% Nonidet P-40 or 0.01% bovine serum albumin Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.140 Fe3+
-
 Streptomyces sp.
3.2.1.140 guanidine hydrochloride
-
 Streptomyces sp.
3.2.1.140 Hg2+
-
 Streptomyces sp.
3.2.1.140 lacto-N-biose
-
 Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.140 additional information
-
 additional information
-
 Streptomyces sp.
3.2.1.140 0.0068
-
 Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-pyridylamine
-
 Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.140 46000
-
 gel filtration Streptomyces sp.
3.2.1.140 60000
-
 1 * 60000, SDS-PAGE Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.140 Streptomyces sp.
-
-
-
3.2.1.140 Streptomyces sp. 142
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.140
-
 Streptomyces sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.140 culture medium
-
 Streptomyces sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.140 18.97
-
-
 Streptomyces sp.

Storage Stability

EC Number Storage Stability Organism
3.2.1.140 4°C or -20°C, protein concentration 0.01 mg/ml or 0.00005 mg/ml, 0.05% Brij 58, stable for at least 6 months Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.140 Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-pyridylamine + H2O
-
 Streptomyces sp. ?
-
 ?
3.2.1.140 Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-pyridylamine + H2O
-
 Streptomyces sp. 142 ?
-
 ?
3.2.1.140 p-nitrophenyl-lacto-N-bioside + H2O
-
 Streptomyces sp. p-nitrophenol + lacto-N-biose
-
 ?
3.2.1.140 p-nitrophenyl-lacto-N-bioside + H2O
-
 Streptomyces sp. 142 p-nitrophenol + lacto-N-biose
-
 ?
3.2.1.140 pyridylamine-oligosaccharides which have type 1 chains at the nonreducing terminus + H2O no reaction with pyridylamine-oligosaccharides which contain only type 2 chains. No reaction with oligosaccharides in which the non-reducing terminal Gal or the penultimate GlcNAc are replaced by fucose or sialic acid are Streptomyces sp. lacto-N-biose + ?
-
 ?
3.2.1.140 pyridylamine-oligosaccharides which have type 1 chains at the nonreducing terminus + H2O no reaction with pyridylamine-oligosaccharides which contain only type 2 chains. No reaction with oligosaccharides in which the non-reducing terminal Gal or the penultimate GlcNAc are replaced by fucose or sialic acid are Streptomyces sp. 142 lacto-N-biose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.140 monomer 1 * 60000, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.140 60
-
 p-nitrophenyl-beta-lacto-N-bioside Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.140 45
-
 30 min, stable Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.140 4.5
-
 p-nitrophenyl-beta-lacto-N-bioside Streptomyces sp.
3.2.1.140 5.5
-
 Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-pyridylamine Streptomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.140 4 10 4°C, 16 h, stable Streptomyces sp.