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Literature summary extracted from

  • Ruile, P.; Winterhalter, C.; Liebl, W.
    Isolation and analysis of a gene encoding alpha-glucuronidase, an enzyme with a novel primary structure involved in the breakdown of xylan (1997), Mol. Microbiol., 23, 267-279.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.139 expression in Escherichia coli Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.139 Ag+
-
Thermotoga maritima
3.2.1.139 Cd2+ 1 mM, strong inhibition Thermotoga maritima
3.2.1.139 Cu2+ 1 mM, strong inhibition Thermotoga maritima
3.2.1.139 Hg2+ 1 mM, strong inhibition Thermotoga maritima
3.2.1.139 p-hydroxymercuribenzoate
-
Thermotoga maritima
3.2.1.139 Pb2+ 1 mM, strong inhibition Thermotoga maritima
3.2.1.139 Zn2+ 1 mM, strong inhibition Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.139 0.95
-
4-O-Methyl-glucuronosyl-xylobiose
-
Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.139 Thermotoga maritima
-
-
-
3.2.1.139 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.139 recombinant enzyme Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.139 4-O-methyl-glucuronosyl-xylobiose + H2O
-
Thermotoga maritima 4-O-methyl-glucuronate + xylobiose
-
?
3.2.1.139 4-O-methyl-glucuronosyl-xylobiose + H2O
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 4-O-methyl-glucuronate + xylobiose
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.139 85
-
-
Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.139 60 100 about 45% of maximal activity at 60°C and at 100°C Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.139 55 75 partial inactivation, but thereafter the residual activity remains nearly constant for several days Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.139 6.3
-
-
Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.139 5 8 pH 5: about 35% of maximal activity, pH 8: about 45% of maximal activity Thermotoga maritima