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Literature summary extracted from

  • Ostoa-Saloma, P.; Garza-Ramos, G.; Ramirez, J.; Becker, I.; Berzunza, M.; Landa, A.; Gomez-Puyou, A.; Tuena de Gomez-Puyou, M.; Perez-Montfort, R.
    Cloning, expression, purification and characterization of triosephosphate isomerase from Trypanosoma cruzi (1997), Eur. J. Biochem., 244, 700-705.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.1
-
Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.1 methyl methanethiosulfonate the sensitivity of enzyme from Trypanosoma cruzi is about 40times higher than that of Trypanosoma brucei and 200times higher than that of Leishmania mexicana Leishmania mexicana
5.3.1.1 methyl methanethiosulfonate the sensitivity of enzyme from Trypanosoma cruzi is about 40times higher than that of Trypanosoma brucei and 200times higher than that of Leishmania mexicana Trypanosoma brucei
5.3.1.1 methyl methanethiosulfonate the sensitivity of enzyme from Trypanosoma cruzi is about 40times higher than that of Trypanosoma brucei and 200times higher than that of Leishmania mexicana Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.1 0.6
-
D-glyceraldehyde 3-phosphate D-glyceraldehyde 3-phosphate Trypanosoma brucei
5.3.1.1 0.9
-
D-glyceraldehyde 3-phosphate
-
Trypanosoma cruzi
5.3.1.1 0.9
-
D-glyceraldehyde 3-phosphate
-
Leishmania mexicana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.1 27244
-
2 * 27244, calculation from nucleotide sequence Trypanosoma cruzi
5.3.1.1 50000
-
gel filtration Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.1 Leishmania mexicana
-
-
-
5.3.1.1 Trypanosoma brucei
-
-
-
5.3.1.1 Trypanosoma cruzi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.1
-
Trypanosoma cruzi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.1 3527
-
-
Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.1 D-Glyceraldehyde 3-phosphate
-
Trypanosoma brucei Glycerone phosphate
-
?
5.3.1.1 D-Glyceraldehyde 3-phosphate
-
Trypanosoma cruzi Glycerone phosphate
-
?
5.3.1.1 D-Glyceraldehyde 3-phosphate
-
Leishmania mexicana Glycerone phosphate
-
?

Subunits

EC Number Subunits Comment Organism
5.3.1.1 dimer
-
Trypanosoma brucei
5.3.1.1 dimer
-
Leishmania mexicana
5.3.1.1 dimer 2 * 27244, calculation from nucleotide sequence Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.1 7 8.5
-
Trypanosoma brucei
5.3.1.1 7 8.5
-
Trypanosoma cruzi
5.3.1.1 7 8.5
-
Leishmania mexicana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.1 6 9 pH 6.0: about 60% of maximal activity, pH 9.0: about 50% of maximal activity Trypanosoma brucei
5.3.1.1 6 9 pH 6.0: about 60% of maximal activity, pH 9.0: about 50% of maximal activity Trypanosoma cruzi
5.3.1.1 6 9 pH 6.0: about 60% of maximal activity, pH 9.0: about 50% of maximal activity Leishmania mexicana