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Literature summary extracted from

  • Kurzok, H.G.; Feierabend, J.
    Comparison of a cytosolic and a chloroplast triosephosphate isomerase isoenzyme from rye leaves. I. Purification and catalytic properties (1984), Biochim. Biophys. Acta, 788, 214-221.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.1 AsO43-
-
 Secale cereale
5.3.1.1 iodoacetate
-
 Secale cereale
5.3.1.1 p-hydroxymercuribenzoate
-
 Secale cereale
5.3.1.1 SO42- CuSO4 Secale cereale

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.1 0.6
-
 D-glyceraldehyde 3-phosphate cytosolic enzyme Secale cereale
5.3.1.1 0.68
-
 D-glyceraldehyde 3-phosphate chloroplastic enzyme Secale cereale
5.3.1.1 1.5
-
 glycerone phosphate glycerone phosphate, cytosolic enzyme Secale cereale
5.3.1.1 2.5
-
 glycerone phosphate chloroplastic enzyme Secale cereale

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.3.1.1 chloroplast
-
 Secale cereale 9507
-
5.3.1.1 cytosol
-
 Secale cereale 5829
-

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.1 Secale cereale
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.1
-
 Secale cereale

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.3.1.1 leaf
-
 Secale cereale
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.1 D-Glyceraldehyde 3-phosphate r Secale cereale Glycerone phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.1 33 40 cytosolic enzyme Secale cereale
5.3.1.1 50
-
 chloroplastic enzyme Secale cereale

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.3.1.1 20 45 20°C: about 65% of maximal activity, 45°C: about 20% of maximal activity, cytosolic enzyme Secale cereale
5.3.1.1 35 53 35°C: about 25% of maximal activity, 53°C: about 70% of maximal activity, chloroplastic enzyme Secale cereale

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.1 40
-
 5 min, cytosolic enzyme, about 50% of activity Secale cereale
5.3.1.1 55
-
 5 min, chloroplastic enzyme, about 30% loss of activity Secale cereale

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.1 7.2 7.5 cytosolic enzyme Secale cereale
5.3.1.1 8.4
-
 chloroplastic enzyme Secale cereale