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Literature summary extracted from

  • Thiebe, R.
    Arginyl-tRNA synthetase from brewer's yeast. Purification, properties, and steady-state mechanism (1983), Eur. J. Biochem., 130, 517-524.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.19 adenosine 5'-[alpha,beta-methylene]triphosphate
-
 Saccharomyces pastorianus
6.1.1.19 Arginyl hydroxamate
-
 Saccharomyces pastorianus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.19 50000
-
 gel filtration, enzyme form II Saccharomyces pastorianus
6.1.1.19 72000
-
 1 * 72000, SDS-PAGE Saccharomyces pastorianus
6.1.1.19 80000
-
 gel filtration, enzyme form I Saccharomyces pastorianus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.19 Saccharomyces pastorianus
-
-
-
6.1.1.19 Saccharomyces pastorianus C836
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.19 two active forms which are interconvertible, I and II Saccharomyces pastorianus

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.19 ATP + L-arginine + tRNAArg = AMP + diphosphate + L-arginyl-tRNAArg simultaneously with the release of AMP or diphosphate an allosteric rearrangement occurs. Cooperative binding of AMP and diphosphate indicates that aminoacylation reaction and backward reaction are concerted Saccharomyces pastorianus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.19 ATP + L-arginine + tRNAArg r Saccharomyces pastorianus AMP + diphosphate + L-arginyl-tRNAArg
-
 ?
6.1.1.19 ATP + L-arginine + tRNAArg recognizes arginine very specifically Saccharomyces pastorianus AMP + diphosphate + L-arginyl-tRNAArg
-
 ?
6.1.1.19 ATP + L-arginine + tRNAArg r Saccharomyces pastorianus C836 AMP + diphosphate + L-arginyl-tRNAArg
-
 ?
6.1.1.19 ATP + L-arginine + tRNAArg recognizes arginine very specifically Saccharomyces pastorianus C836 AMP + diphosphate + L-arginyl-tRNAArg
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.19 monomer 1 * 72000, SDS-PAGE Saccharomyces pastorianus