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Literature summary extracted from
- Glutaminyl-tRNA synthetase of Escherichia coli (1985), Methods Enzymol., 113, 55-59.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 6.1.1.18 | 0.0001 | - |
tRNAGln in unfractionated tRNA | - |
Escherichia coli | |
| 6.1.1.18 | 0.11 | - |
Gln | Gln | Escherichia coli |  |
| 6.1.1.18 | 0.111 | - |
ATP | - |
Escherichia coli | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.1.1.18 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 6.1.1.18 | - |
Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 6.1.1.18 | 0.1074 | - |
- |
Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 6.1.1.18 | -20°C, 20 mM potassium phosphate, pH 7.5, 50 mM KCl, 1 mM DTT, 50% glycerol, stable for 6 months | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.1.1.18 | ATP + L-glutamine + tRNAGln | - |
Escherichia coli | AMP + diphosphate + L-glutaminyl-tRNAGln | - |
? | |
| 6.1.1.18 | ATP + L-glutamine + tRNAGln in unfractionated tRNA | - |
Escherichia coli | ? | - |
? | |
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