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Literature summary extracted from

  • Randez-Gil, F.; Blasco, A.; Prieto, J.A.; Sanz, P.
    DOGR1 and DOGR2: two genes from Saccharomyces cerevisiae that confer 2-deoxyglucose resistance when overexpressed (1995), Yeast, 11, 1233-1240.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.68 phosphate
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.68 17
-
 2-deoxy-D-glucose 6-phosphate isoenzyme DogR1p Saccharomyces cerevisiae
3.1.3.68 41
-
 2-deoxy-D-glucose 6-phosphate isoenzyme DogR2p Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.68 Mg2+ required, maximal activation with 10 mM Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.68 Saccharomyces cerevisiae
-
 the enzyme is also present in wild type yeast, although its level is ten to twenty times lower than those found in the mutant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.68 2 isoenzymes: DogR1p and DogR2p Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.68 2-Deoxy-D-glucose 6-phosphate + H2O
-
 Saccharomyces cerevisiae 2-Deoxy-D-glucose + phosphate
-
 ?
3.1.3.68 2-Deoxyribose 5-phosphate + H2O isoenzyme DogR1p: at 21% of the activity with 2-deoxy-D-glucose 6-phosphate, isoenzyme DogR2p: at 1% of the activity with 2-deoxy-D-glucose 6-phosphate Saccharomyces cerevisiae 2-Deoxyribose + phosphate
-
 ?
3.1.3.68 Arabinose 5-phosphate + H2O isoenzyme DogR1p: at 6% of the activity with 2-deoxy-D-glucose 6-phosphate, isoenzyme DogR2p: at 6% of the activity with 2-deoxy-D-glucose 6-phosphate Saccharomyces cerevisiae Arabinose + phosphate
-
 ?
3.1.3.68 D-fructose 6-phosphate + H2O isoenzyme DogR1p: at 2% of the activity with 2-deoxy-D-glucose 6-phosphate, isoenzyme DogR2p: at 17% of the activity with 2-deoxy-D-glucose 6-phosphate Saccharomyces cerevisiae D-fructose + phosphate
-
 ?
3.1.3.68 D-ribose 5-phosphate + H2O isoenzyme DogR1p: at 42% of the activity with 2-deoxy-D-glucose 6-phosphate, isoenzyme DogR2p: at 13% of the activity with 2-deoxy-D-glucose 6-phosphate Saccharomyces cerevisiae D-ribose + phosphate
-
 ?
3.1.3.68 Fructose 1-phosphate + H2O isoenzyme DogR1p: at 12% of the activity with 2-deoxy-D-glucose 6-phosphate, isoenzyme DogR2p: at 106% of the activity with 2-deoxy-D-glucose 6-phosphate Saccharomyces cerevisiae Fructose + phosphate
-
 ?
3.1.3.68 Glucose 6-phosphate + H2O isoenzyme DogR1p: at 20% of the activity with 2-deoxy-D-glucose 6-phosphate, isoenzyme DogR2p: at 30% of the activity with 2-deoxy-D-glucose 6-phosphate Saccharomyces cerevisiae Glucose + phosphate
-
 ?
3.1.3.68 Ribulose 5-phosphate + H2O isoenzyme DogR1p: at 7% of the activity with 2-deoxy-D-glucose 6-phosphate, isoenzyme DogR2p: at 1% of the activity with 2-deoxy-D-glucose 6-phosphate Saccharomyces cerevisiae Ribulose + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.68 30
-
 isoenzyme DogR1p Saccharomyces cerevisiae
3.1.3.68 40
-
 isoenzyme DogR2p Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.68 6
-
 isoenzyme DogR1p and DogR2p Saccharomyces cerevisiae