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Literature summary extracted from

  • Singer, M.F.; Fruton, J.S.
    Some properties of beef spleen phosphoamidase (1957), J. Biol. Chem., 229, 111-119.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.9.1.1 sulfhydryl compounds i.e. beta-mercaptoethanol Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.9.1.1 Fe2+ 40% inhibition Bos taurus
3.9.1.1 Fe3+ 30% inhibition Bos taurus
3.9.1.1 fluoride
-
 Bos taurus
3.9.1.1 Iodosobenzene
-
 Bos taurus
3.9.1.1 p-chloromercuribenzoate
-
 Bos taurus
3.9.1.1 phosphate concentration higher than 1 mM Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.9.1.1 creatine phosphate + H2O Bos taurus
-
 creatine + o-phosphate
-
 ?
3.9.1.1 phosphoramidate + H2O Bos taurus cleavage of the N-P bond NH4+ + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.9.1.1 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.9.1.1 50fold Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.9.1.1 N-phosphocreatine + H2O = creatine + phosphate also acts on N-phospho-arginine and other phosphoamides Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.9.1.1 spleen
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.9.1.1 additional information
-
 specific activity 38 enzyme units per mg protein with substrate o-carboxyphenylphosphate, 1 enzyme unit defined as amount of enzyme causing absorbance change per 5 min, measurement of salicylic acid Bos taurus
3.9.1.1 2.2
-
 substrate phosvitin, measurement of phosphate Bos taurus
3.9.1.1 4.8
-
 substrate phosphoramidate, measurement of NH4+ Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.9.1.1 -18°C, lyophilized, 1 month Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.9.1.1 alpha-N-phosphoryl-DL-tryptophan methyl ester + H2O
-
 Bos taurus DL-tryptophan methyl ester + phosphate
-
 ?
3.9.1.1 creatine phosphate + H2O
-
 Bos taurus creatine + phosphate
-
 ?
3.9.1.1 creatine phosphate + H2O
-
 Bos taurus creatine + o-phosphate
-
 ?
3.9.1.1 N-(p-chlorophenyl)phosphoramidate + H2O
-
 Bos taurus 4-chlorophenylamine + phosphate
-
 ?
3.9.1.1 N-(p-tolyl)phosphoramidate + H2O
-
 Bos taurus 4-aminophenol + phosphate
-
 ?
3.9.1.1 N-phosphoglycine + H2O
-
 Bos taurus glycine + phosphate
-
 ?
3.9.1.1 o-carboxyphenylphosphate + H2O
-
 Bos taurus salicylic acid + phosphate + CO2
-
 ?
3.9.1.1 O-phospho-L-tyrosine + H2O
-
 Bos taurus L-tyrosine + phosphate
-
 ?
3.9.1.1 p-nitrophenylphosphate + H2O
-
 Bos taurus p-nitrophenol + phosphate
-
 ?
3.9.1.1 phosphoramidate + H2O cleavage of the N-P bond Bos taurus NH4+ + phosphate
-
 ?
3.9.1.1 phosvitin + H2O
-
 Bos taurus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.9.1.1 70
-
 heating for 5 min does not destroy the enzyme activity or precipitate the enzyme Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.9.1.1 6
-
 about Bos taurus