EC Number | Application | Comment | Organism |
---|---|---|---|
3.8.1.8 | agriculture | degradation of chemically stable and toxic herbicide atrazine in soil and groundwater to non-toxic hydroxyatrazine | Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.8.1.8 | 0.149 | - |
Atrazine | - |
Pseudomonas sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.8.1.8 | additional information | no metal ions detectable with the enzym expressed in E. coli | Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.8.1.8 | 245000 | - |
gel filtration | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.8.1.8 | atrazine + H2O | Pseudomonas sp. | - |
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.8.1.8 | Pseudomonas sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.8.1.8 | - |
Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.8.1.8 | 4.5 | - |
purified enzyme | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.8.1.8 | atrazine + H2O | - |
Pseudomonas sp. | 4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl | - |
? | |
3.8.1.8 | desethylatrazine + H2O | - |
Pseudomonas sp. | 2-hydroxy-4-amino-6-(isopropylamino)-1,3,5-triazine + HCl | - |
? | |
3.8.1.8 | simazine + H2O | - |
Pseudomonas sp. | 4,6-diethylamino-1,3,5-triazine + HCl | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.8.1.8 | pentamer | 4 or 5 * 60000-52400, SDS-PAGE and calculation from DNA-sequence | Pseudomonas sp. |
3.8.1.8 | tetramer | 4 or 5 * 60000-52400, SDS-PAGE and calculation from DNA-sequence | Pseudomonas sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.8.1.8 | AtzA | - |
- |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.8.1.8 | 11 | - |
Atrazine | - |
Pseudomonas sp. |