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Literature summary extracted from

  • Nagata, Y.; Miyauchi, K.; Damborsky, J.; Manova, K.; Ansorgova, A.; Takagi, M.
    Purification and characterization of a haloalkane dehalogenase of a new substrate class from a gamma-hexachlorocyclohexane-degrading bacterium, Sphingomonas paucimobilis UT26 (1997), Appl. Environ. Microbiol., 63, 3707-3710.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.8.1.5 agriculture biodegradation of pollutant insecticide gamma-hexachlorocyclohexane (lindane) Sphingomonas paucimobilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.5 UT26, overexpression in Escherichia coli Sphingomonas paucimobilis

Protein Variants

EC Number Protein Variants Comment Organism
3.8.1.5 additional information UT26, W175 probably naturally exchanged for F or Q explaining substrate specificities Sphingomonas paucimobilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.8.1.5 32000
-
 gel filtration, SDS-PAGE Sphingomonas paucimobilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.5 1,3,4,6-tetrachloro-1,4,cyclohexadiene + 2 H2O Sphingomonas paucimobilis
-
 2,5-dichloro-2,5-cyclohexadiene-1,4-diol + 2 chloride
-
 ?
3.8.1.5 n-haloalkanes + H2O Sphingomonas paucimobilis broad substrate range primary alcohols + halide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.5 Sphingomonas paucimobilis
-
 e.g. Pseudomonas paucimobilis
-
3.8.1.5 Sphingomonas paucimobilis
-
 UT26
-
3.8.1.5 Sphingomonas paucimobilis UT26
-
 UT26
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.5 UT26 Sphingomonas paucimobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.5 1,2-dibromoethane + H2O
-
 Sphingomonas paucimobilis 2-bromoethanol + bromide
-
 ?
3.8.1.5 1,2-dibromoethane + H2O
-
 Sphingomonas paucimobilis UT26 2-bromoethanol + bromide
-
 ?
3.8.1.5 1,2-dichloroethane + H2O
-
 Sphingomonas paucimobilis 2-chloroethanol + chloride
-
 ?
3.8.1.5 1,2-dichloroethane + H2O
-
 Sphingomonas paucimobilis UT26 2-chloroethanol + chloride
-
 ?
3.8.1.5 1,3,4,6-tetrachloro-1,4,cyclohexadiene + 2 H2O
-
 Sphingomonas paucimobilis 2,5-dichloro-2,5-cyclohexadiene-1,4-diol + 2 chloride
-
 ?
3.8.1.5 1-chloro-2-bromoethane + H2O
-
 Sphingomonas paucimobilis ?
-
 ?
3.8.1.5 1-chlorobutane + H2O
-
 Sphingomonas paucimobilis butanol + chloride
-
 ?
3.8.1.5 1-chlorobutane + H2O
-
 Sphingomonas paucimobilis UT26 butanol + chloride
-
 ?
3.8.1.5 haloalkane + H2O broad specificity Sphingomonas paucimobilis alcohol + halide
-
 ?
3.8.1.5 haloalkane + H2O alpha,omega-dihalogenated ethanes, preference for long-chain haloalkanes Sphingomonas paucimobilis alcohol + halide
-
 ?
3.8.1.5 haloalkane + H2O broad specificity Sphingomonas paucimobilis UT26 alcohol + halide
-
 ?
3.8.1.5 haloalkane + H2O alpha,omega-dihalogenated ethanes, preference for long-chain haloalkanes Sphingomonas paucimobilis UT26 alcohol + halide
-
 ?
3.8.1.5 n-haloalkanes + H2O broad substrate range Sphingomonas paucimobilis primary alcohols + halide
-
 ?

Subunits

EC Number Subunits Comment Organism
3.8.1.5 monomer 1 * 32000, SDS-PAGE Sphingomonas paucimobilis

Synonyms

EC Number Synonyms Comment Organism
3.8.1.5 1,3,4,6,-tetrachloro-1,4-cyclohexadiene halidohydrolase
-
 Sphingomonas paucimobilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.5 8.2
-
-
 Sphingomonas paucimobilis