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Literature summary extracted from

  • Sallis, P.J.; Armfield, S.J.; Bull, A.T.; Hardman, D.J.
    Isolation and characterization of a haloalkane halidohydrolase from Rhodococcus erythropolis Y2 (1990), J. Gen. Microbiol., 136, 115-120.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.8.1.5 CaCl2
-
 Rhodococcus erythropolis
3.8.1.5 CuSO4
-
 Rhodococcus erythropolis
3.8.1.5 Dibromomethane
-
 Rhodococcus erythropolis
3.8.1.5 Dichloromethane
-
 Rhodococcus erythropolis
3.8.1.5 FeSO4
-
 Rhodococcus erythropolis
3.8.1.5 HgCl2
-
 Rhodococcus erythropolis
3.8.1.5 NEM
-
 Rhodococcus erythropolis
3.8.1.5 NiSO4
-
 Rhodococcus erythropolis
3.8.1.5 ZnSO4
-
 Rhodococcus erythropolis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.5 0.26
-
 1-Chlorobutane
-
 Rhodococcus erythropolis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.8.1.5 34000
-
 gel filtration Rhodococcus erythropolis

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.5 Rhodococcus erythropolis
-
 Y2
-
3.8.1.5 Rhodococcus erythropolis Y2
-
 Y2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.5 strain Y2 Rhodococcus erythropolis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8.1.5 additional information
-
-
 Rhodococcus erythropolis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.5 1-chlorobutane + H2O
-
 Rhodococcus erythropolis butanol + chloride
-
 ?
3.8.1.5 1-chlorobutane + H2O
-
 Rhodococcus erythropolis Y2 butanol + chloride
-
 ?
3.8.1.5 haloalkane + H2O overview Rhodococcus erythropolis alcohol + halide
-
 ?
3.8.1.5 haloalkane + H2O broad specificity Rhodococcus erythropolis alcohol + halide
-
 ?
3.8.1.5 haloalkane + H2O highest activity towards alpha,omega disubstituted chloro-C2-C6 and bromo-C2-C6 alkanes and 4-chlorobutanol Rhodococcus erythropolis alcohol + halide
-
 ?
3.8.1.5 haloalkane + H2O overview Rhodococcus erythropolis Y2 alcohol + halide
-
 ?
3.8.1.5 haloalkane + H2O broad specificity Rhodococcus erythropolis Y2 alcohol + halide
-
 ?
3.8.1.5 haloalkane + H2O highest activity towards alpha,omega disubstituted chloro-C2-C6 and bromo-C2-C6 alkanes and 4-chlorobutanol Rhodococcus erythropolis Y2 alcohol + halide
-
 ?

Subunits

EC Number Subunits Comment Organism
3.8.1.5 monomer 1 * 34000, SDS-PAGE Rhodococcus erythropolis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.5 9.2
-
-
 Rhodococcus erythropolis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.8.1.5 7.5 11 more than 50% of maximal activity at pH 7.5 and 11.0 Rhodococcus erythropolis