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Literature summary extracted from

  • Duggleby, C.J.; Williams, P.A.
    Purification and some properties of the 2-hydroxy-6-oxohepta-2,4-dienoate hydrolase (2-hydroxymuconic semialdehyde hydrolase) encoded by the TOL plasmid pWW0 from Pseudomonas putida mt-2 (1986), J. Gen. Microbiol., 132, 717-726.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.7.1.9 AgNO3
-
 Pseudomonas putida
3.7.1.9 CuCl2
-
 Pseudomonas putida
3.7.1.9 iodoacetamide
-
 Pseudomonas putida
3.7.1.9 PCMB
-
 Pseudomonas putida
3.7.1.9 sodium arsenite
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.9 0.005
-
 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-propylcatechol
-
 Pseudomonas putida
3.7.1.9 0.01
-
 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-methylcatechol
-
 Pseudomonas putida
3.7.1.9 0.015
-
 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-ethylcatechol
-
 Pseudomonas putida
3.7.1.9 0.022
-
 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-ethylcatechol
-
 Pseudomonas putida
3.7.1.9 0.023
-
 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-propylcatechol
-
 Pseudomonas putida
3.7.1.9 0.03
-
 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon catechol
-
 Pseudomonas putida
3.7.1.9 0.036
-
 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-methylcatechol
-
 Pseudomonas putida
3.7.1.9 0.037
-
 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-allylcatechol
-
 Pseudomonas putida
3.7.1.9 0.122
-
 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-chlorocatechol
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.7.1.9 65000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.7.1.9 additional information Pseudomonas putida enzyme is part of the pathway for the catabolism of toluene and m-xylene and p-xylene ?
-
 ?
3.7.1.9 additional information Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 enzyme is part of the pathway for the catabolism of toluene and m-xylene and p-xylene ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.9 Pseudomonas putida
-
-
-
3.7.1.9 Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.9
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7.1.9 48
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.9 2-hydroxy-6-oxohepta-2,4-dienoate + H2O
-
 Pseudomonas putida ?
-
 ?
3.7.1.9 2-hydroxy-6-oxohepta-2,4-dienoate + H2O
-
 Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 ?
-
 ?
3.7.1.9 2-hydroxymuconate semialdehyde + H2O
-
 Pseudomonas putida formate + 2-oxopent-4-enoate
-
 ?
3.7.1.9 2-hydroxymuconate semialdehyde + H2O
-
 Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 formate + 2-oxopent-4-enoate
-
 ?
3.7.1.9 3-allylcatechol ring-fission product + H2O meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-allylcatechol Pseudomonas putida ?
-
 ?
3.7.1.9 3-ethylcatechol ring-fission product + H2O meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-ethylcatechol Pseudomonas putida ?
-
 ?
3.7.1.9 3-methylcatechol ring-fission product + H2O meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-methylcatechol Pseudomonas putida ?
-
 ?
3.7.1.9 3-methylcatechol ring-fission product + H2O meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-methylcatechol Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 ?
-
 ?
3.7.1.9 3-propylcatechol ring-fission product + H2O meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-propylcatechol Pseudomonas putida ?
-
 ?
3.7.1.9 4-chlorocatechol ring-fission product + H2O meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-chlorocatechol Pseudomonas putida ?
-
 ?
3.7.1.9 4-ethylcatechol ring-fission product + H2O meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-ethylcatechol Pseudomonas putida ?
-
 ?
3.7.1.9 4-methylcatechol ring-fission product + H2O meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-methylcatechol Pseudomonas putida ?
-
 ?
3.7.1.9 4-propylcatechol ring-fission product + H2O meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-propylcatechol Pseudomonas putida ?
-
 ?
3.7.1.9 catechol ring-fission product + H2O meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon catechol Pseudomonas putida ?
-
 ?
3.7.1.9 catechol ring-fission product + H2O meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon catechol Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 ?
-
 ?
3.7.1.9 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-allylcatechol + H2O
-
 Pseudomonas putida ?
-
 ?
3.7.1.9 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-ethylcatechol + H2O
-
 Pseudomonas putida ?
-
 ?
3.7.1.9 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-methylcatechol + H2O
-
 Pseudomonas putida ?
-
 ?
3.7.1.9 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 3-propylcatechol + H2O
-
 Pseudomonas putida ?
-
 ?
3.7.1.9 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-chlorocatechol + H2O
-
 Pseudomonas putida ?
-
 ?
3.7.1.9 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-ethylcatechol + H2O
-
 Pseudomonas putida ?
-
 ?
3.7.1.9 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-methylcatechol + H2O
-
 Pseudomonas putida ?
-
 ?
3.7.1.9 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon 4-propylcatechol + H2O
-
 Pseudomonas putida ?
-
 ?
3.7.1.9 meta-cleavage product resulting from the action of catechol 2,3-oxygenase upon catechol + H2O
-
 Pseudomonas putida ?
-
 ?
3.7.1.9 additional information enzyme is part of the pathway for the catabolism of toluene and m-xylene and p-xylene Pseudomonas putida ?
-
 ?
3.7.1.9 additional information enzyme is part of the pathway for the catabolism of toluene and m-xylene and p-xylene Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.7.1.9 dimer 2 * 31000-33000, SDS-PAGE after denaturation with 2-mercaptoethanol and urea Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7.1.9 7 7.5
-
 Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.7.1.9 7 8 optimal stability at pH 7-8, rapid loss of activity below pH 5 and above pH 9.5 Pseudomonas putida