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Literature summary extracted from

  • King, S.M.; Otter, T.; Witman, G.B.
    Purification and characterization of Chlamydomonas flagellar dyneins (1986), Methods Enzymol., 134, 291-306.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.1.2 flagellum of sperms Chlamydomonas reinhardtii
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Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.1.2 16000
-
 alpha,LC1, 1 * 440000 + 1 * 16000, 18S dynein, alpha subunit Chlamydomonas reinhardtii
5.6.1.2 18000
-
 alpha,LC1,LC2, 1 * 400000 + 1 * 22000 + 1 * 18000, 12S dynein Chlamydomonas reinhardtii
5.6.1.2 22000
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 alpha,LC1,LC2, 1 * 400000 + 1 * 22000 + 1 * 18000, 12S dynein Chlamydomonas reinhardtii
5.6.1.2 69000
-
 alpha, IC1,IC2,LC1-7, 1 * 420000 + 1 * 78000 + 1 * 69000 + 7 * 8000-20000, 18S dynein, beta subunit Chlamydomonas reinhardtii
5.6.1.2 78000
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 alpha, IC1,IC2,LC1-7, 1 * 420000 + 1 * 78000 + 1 * 69000 + 7 * 8000-20000, 18S dynein, beta subunit Chlamydomonas reinhardtii
5.6.1.2 400000
-
 alpha,LC1,LC2, 1 * 400000 + 1 * 22000 + 1 * 18000, 12S dynein Chlamydomonas reinhardtii
5.6.1.2 420000
-
 alpha, IC1,IC2,LC1-7, 1 * 420000 + 1 * 78000 + 1 * 69000 + 7 * 8000-20000, 18S dynein, beta subunit Chlamydomonas reinhardtii
5.6.1.2 440000
-
 alpha,LC1, 1 * 440000 + 1 * 16000, 18S dynein, alpha subunit Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.1.2 ATP + H2O + a dynein associated with a microtubule at position n Chlamydomonas reinhardtii
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 ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.2 Chlamydomonas reinhardtii
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Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.2 extraction of axonemes with high salt, 0.6 M KCl, 5-20% sucrose gradient centrifugation, hydroxylapatite Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.2 ATP + H2O + a dynein associated with a microtubule at position n
-
 Chlamydomonas reinhardtii ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Subunits

EC Number Subunits Comment Organism
5.6.1.2 oligomer alpha,LC1, 1 * 440000 + 1 * 16000, 18S dynein, alpha subunit Chlamydomonas reinhardtii
5.6.1.2 oligomer alpha, IC1,IC2,LC1-7, 1 * 420000 + 1 * 78000 + 1 * 69000 + 7 * 8000-20000, 18S dynein, beta subunit Chlamydomonas reinhardtii
5.6.1.2 trimer alpha,LC1,LC2, 1 * 400000 + 1 * 22000 + 1 * 18000, 12S dynein Chlamydomonas reinhardtii