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Literature summary extracted from

  • Stephens, R.E.; Prior, G.
    Dynein from serotonin-activated cilia and flagella: extraction characteristics and distinct sites for cAMP-dependent protein phosphorylation (1992), J. Cell Sci., 103, 999-1012.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.1.2 cilium
-
 Mytilus edulis 5929
-
5.6.1.2 cilium
-
 Spisula solidissima 5929
-
5.6.1.2 flagellum of sperms Mytilus edulis
-
-
5.6.1.2 flagellum of sperms Spisula solidissima
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.1.2 14000
-
 alpha1,alpha2,IC2,IC3,LC2,LC3,LC4, 1 * 450000 + 1 * 430000 + 1 * 96000 + 1 * 82000 + 1* 18000 + 1 * 16500 + 1 * 14000, dynein polypeptides from flagella, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Mytilus edulis
5.6.1.2 14500
-
 alpha1,alpha2,IC1,IC2,IC3,LC2,LC3,LC4, 1 * 450000 + 1 * 430000 + 1 * 130000 + 1 * 96000 + 1 * 82500 + 1* 19000 + 1 * 16500 + 1 * 14500, dynein polypeptides from flagella, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Spisula solidissima
5.6.1.2 16500
-
 alpha1,alpha2,IC2,IC3,LC2,LC3,LC4, 1 * 450000 + 1 * 430000 + 1 * 96000 + 1 * 82000 + 1* 18000 + 1 * 16500 + 1 * 14000, dynein polypeptides from flagella, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Mytilus edulis
5.6.1.2 16500
-
 alpha1,alpha2,IC1,IC2,IC3,LC2,LC3,LC4, 1 * 450000 + 1 * 430000 + 1 * 130000 + 1 * 96000 + 1 * 82500 + 1* 19000 + 1 * 16500 + 1 * 14500, dynein polypeptides from flagella, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Spisula solidissima
5.6.1.2 17000
-
 alpha1,alpha2,IC2,IC3,LC1,LC2,LC3, 1 * 450000 + 1 * 430000 + 1 * 94000 + 1 * 80000 + 1* 27000 + 1 * 21000 + 1 * 17000, dynein polypeptides from cilia, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Mytilus edulis
5.6.1.2 17000
-
 alpha1,alpha2,IC2,IC3,LC2,LC3, 1 * 450000 + 1 * 430000 + 1 * 90000 + 1 * 78000 + 1* 20000 + 1 * 17000, dynein polypeptides from cilia, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Spisula solidissima
5.6.1.2 21000
-
 alpha1,alpha2,IC2,IC3,LC1,LC2,LC3, 1 * 450000 + 1 * 430000 + 1 * 94000 + 1 * 80000 + 1* 27000 + 1 * 21000 + 1 * 17000, dynein polypeptides from cilia, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Mytilus edulis
5.6.1.2 78000
-
 alpha1,alpha2,IC2,IC3,LC2,LC3, 1 * 450000 + 1 * 430000 + 1 * 90000 + 1 * 78000 + 1* 20000 + 1 * 17000, dynein polypeptides from cilia, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Spisula solidissima
5.6.1.2 80000
-
 alpha1,alpha2,IC2,IC3,LC1,LC2,LC3, 1 * 450000 + 1 * 430000 + 1 * 94000 + 1 * 80000 + 1* 27000 + 1 * 21000 + 1 * 17000, dynein polypeptides from cilia, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Mytilus edulis
5.6.1.2 82000
-
 alpha1,alpha2,IC2,IC3,LC2,LC3,LC4, 1 * 450000 + 1 * 430000 + 1 * 96000 + 1 * 82000 + 1* 18000 + 1 * 16500 + 1 * 14000, dynein polypeptides from flagella, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Mytilus edulis
5.6.1.2 82500
-
 alpha1,alpha2,IC1,IC2,IC3,LC2,LC3,LC4, 1 * 450000 + 1 * 430000 + 1 * 130000 + 1 * 96000 + 1 * 82500 + 1* 19000 + 1 * 16500 + 1 * 14500, dynein polypeptides from flagella, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Spisula solidissima
5.6.1.2 90000
-
 alpha1,alpha2,IC2,IC3,LC2,LC3, 1 * 450000 + 1 * 430000 + 1 * 90000 + 1 * 78000 + 1* 20000 + 1 * 17000, dynein polypeptides from cilia, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Spisula solidissima
5.6.1.2 94000
-
 alpha1,alpha2,IC2,IC3,LC1,LC2,LC3, 1 * 450000 + 1 * 430000 + 1 * 94000 + 1 * 80000 + 1* 27000 + 1 * 21000 + 1 * 17000, dynein polypeptides from cilia, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Mytilus edulis
5.6.1.2 96000
-
 alpha1,alpha2,IC2,IC3,LC2,LC3,LC4, 1 * 450000 + 1 * 430000 + 1 * 96000 + 1 * 82000 + 1* 18000 + 1 * 16500 + 1 * 14000, dynein polypeptides from flagella, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Mytilus edulis
5.6.1.2 96000
-
 alpha1,alpha2,IC1,IC2,IC3,LC2,LC3,LC4, 1 * 450000 + 1 * 430000 + 1 * 130000 + 1 * 96000 + 1 * 82500 + 1* 19000 + 1 * 16500 + 1 * 14500, dynein polypeptides from flagella, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Spisula solidissima
5.6.1.2 130000
-
 alpha1,alpha2,IC1,IC2,IC3,LC2,LC3,LC4, 1 * 450000 + 1 * 430000 + 1 * 130000 + 1 * 96000 + 1 * 82500 + 1* 19000 + 1 * 16500 + 1 * 14500, dynein polypeptides from flagella, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Spisula solidissima
5.6.1.2 430000
-
 alpha1,alpha2,IC2,IC3,LC2,LC3,LC4, 1 * 450000 + 1 * 430000 + 1 * 96000 + 1 * 82000 + 1* 18000 + 1 * 16500 + 1 * 14000, dynein polypeptides from flagella, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Mytilus edulis
5.6.1.2 430000
-
 alpha1,alpha2,IC2,IC3,LC1,LC2,LC3, 1 * 450000 + 1 * 430000 + 1 * 94000 + 1 * 80000 + 1* 27000 + 1 * 21000 + 1 * 17000, dynein polypeptides from cilia, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Mytilus edulis
5.6.1.2 430000
-
 alpha1,alpha2,IC1,IC2,IC3,LC2,LC3,LC4, 1 * 450000 + 1 * 430000 + 1 * 130000 + 1 * 96000 + 1 * 82500 + 1* 19000 + 1 * 16500 + 1 * 14500, dynein polypeptides from flagella, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Spisula solidissima
5.6.1.2 430000
-
 alpha1,alpha2,IC2,IC3,LC2,LC3, 1 * 450000 + 1 * 430000 + 1 * 90000 + 1 * 78000 + 1* 20000 + 1 * 17000, dynein polypeptides from cilia, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Spisula solidissima
5.6.1.2 450000
-
 alpha1,alpha2,IC2,IC3,LC2,LC3,LC4, 1 * 450000 + 1 * 430000 + 1 * 96000 + 1 * 82000 + 1* 18000 + 1 * 16500 + 1 * 14000, dynein polypeptides from flagella, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Mytilus edulis
5.6.1.2 450000
-
 alpha1,alpha2,IC2,IC3,LC1,LC2,LC3, 1 * 450000 + 1 * 430000 + 1 * 94000 + 1 * 80000 + 1* 27000 + 1 * 21000 + 1 * 17000, dynein polypeptides from cilia, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Mytilus edulis
5.6.1.2 450000
-
 alpha1,alpha2,IC1,IC2,IC3,LC2,LC3,LC4, 1 * 450000 + 1 * 430000 + 1 * 130000 + 1 * 96000 + 1 * 82500 + 1* 19000 + 1 * 16500 + 1 * 14500, dynein polypeptides from flagella, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Spisula solidissima
5.6.1.2 450000
-
 alpha1,alpha2,IC2,IC3,LC2,LC3, 1 * 450000 + 1 * 430000 + 1 * 90000 + 1 * 78000 + 1* 20000 + 1 * 17000, dynein polypeptides from cilia, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Spisula solidissima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.1.2 ATP + H2O + a dynein associated with a microtubule at position n Mytilus edulis
-
 ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.2 ATP + H2O + a dynein associated with a microtubule at position n Spisula solidissima
-
 ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.2 Mytilus edulis
-
 blue mussel
-
5.6.1.2 Spisula solidissima
-
 surf clam
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
5.6.1.2 side-chain modification cyclic AMP-dependent phosphorylation takes place on specific light chains in cilia but only on alpha heavy chains in flagella, phosphorylation has no effect on steady-state ATPase activity Mytilus edulis
5.6.1.2 side-chain modification cyclic AMP-dependent phosphorylation takes place on specific light chains in cilia but only on alpha heavy chains in flagella, phosphorylation has no effect on steady-state ATPase activity Spisula solidissima

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.2 high-salt extraction, sucrose gradient Mytilus edulis
5.6.1.2 high-salt extraction, sucrose gradient Spisula solidissima

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.1.2 semen
-
 Mytilus edulis
-
5.6.1.2 semen
-
 Spisula solidissima
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6.1.2 0.14
-
 sperm flagellar axonemes Mytilus edulis
5.6.1.2 0.62
-
 salt extract with detergent Mytilus edulis
5.6.1.2 0.72
-
 salt extract Mytilus edulis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.2 ATP + H2O + a dynein associated with a microtubule at position n
-
 Mytilus edulis ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.2 ATP + H2O + a dynein associated with a microtubule at position n
-
 Spisula solidissima ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Subunits

EC Number Subunits Comment Organism
5.6.1.2 oligomer alpha1,alpha2,IC2,IC3,LC2,LC3,LC4, 1 * 450000 + 1 * 430000 + 1 * 96000 + 1 * 82000 + 1* 18000 + 1 * 16500 + 1 * 14000, dynein polypeptides from flagella, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Mytilus edulis
5.6.1.2 oligomer alpha1,alpha2,IC2,IC3,LC1,LC2,LC3, 1 * 450000 + 1 * 430000 + 1 * 94000 + 1 * 80000 + 1* 27000 + 1 * 21000 + 1 * 17000, dynein polypeptides from cilia, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Mytilus edulis
5.6.1.2 oligomer alpha1,alpha2,IC1,IC2,IC3,LC2,LC3,LC4, 1 * 450000 + 1 * 430000 + 1 * 130000 + 1 * 96000 + 1 * 82500 + 1* 19000 + 1 * 16500 + 1 * 14500, dynein polypeptides from flagella, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Spisula solidissima
5.6.1.2 oligomer alpha1,alpha2,IC2,IC3,LC2,LC3, 1 * 450000 + 1 * 430000 + 1 * 90000 + 1 * 78000 + 1* 20000 + 1 * 17000, dynein polypeptides from cilia, heavy chains, intermediate chains (IC) and light chains (LC), SDS-PAGE Spisula solidissima