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Literature summary extracted from

  • Romac, P.; Zanic-Grubisic, T.; Culic, O.; Cvitkovic, P.; Flogel, M.
    Sperm motility and kinetics of dynein ATPase in astheno- and normozoospermic samples after stimulation with adenosine and its analogues (1994), Hum. Reprod., 9, 1474-1478.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.1.2 EDTA ATP hydrolysis completely blocked after preincubation with 2 mM EDTA for 30 min Homo sapiens
5.6.1.2 VO43- 60% activity after preincubation with 0.010 mM vanadate for 30 min, vanadate acts as phosphate analog that binds to the gamma-phosphate locus in the ATP-binding site Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.6.1.2 0.12
-
 ATP asthenozoospermic samples Homo sapiens
5.6.1.2 0.13
-
 ATP normozoospermic samples Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.1.2 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.1.2 ATP + H2O + a dynein associated with a microtubule at position n Homo sapiens
-
 ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.2 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.1.2 semen
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6.1.2 0.007
-
 asthenozoospermic samples Homo sapiens
5.6.1.2 0.012
-
 asthenozoospermic samples, addition of 0.001 mM 5'-(N-ethylcarboxyamido)-adenosine Homo sapiens
5.6.1.2 0.013
-
 asthenozoospermic samples, addition of 2 mM adenosine Homo sapiens
5.6.1.2 0.013
-
 asthenozoospermic samples, addition of 2 mM adenosine and 50 microM dipyridamol Homo sapiens
5.6.1.2 0.014
-
 normozoospermic samples Homo sapiens
5.6.1.2 0.014
-
 asthenozoospermic samples, addition of 2 mM 2-deoxyadenosine Homo sapiens
5.6.1.2 0.018
-
 normozoospermic samples, addition of 2 mM adenosine Homo sapiens
5.6.1.2 0.018
-
 normozoospermic samples, addition of 0.001 mM 5'-(N-ethylcarboxyamido)-adenosine Homo sapiens
5.6.1.2 0.018
-
 normozoospermic samples, addition of 2 mM adenosine and 0.05 mM dipyridamol Homo sapiens
5.6.1.2 0.019
-
 normozoospermic samples, addition of 2 mM 2-deoxyadenosine Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.2 ATP + H2O + a dynein associated with a microtubule at position n
-
 Homo sapiens ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?