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Literature summary extracted from
- H+-ATPase from plasma membranes of Saccharomyces cerevisiae and Avena sativa roots: purification and reconstitution (1988), Methods Enzymol., 157, 533-544.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.2.1 | NH4+ | small activation | Saccharomyces cerevisiae |  |
| 7.1.2.1 | NH4+ | small activation | Avena sativa | |
| 7.1.2.1 | Phospholipid | activated 3fold to 6fold by soybean phospholipids | Saccharomyces cerevisiae | |
| 7.1.2.1 | Phospholipid | activated by exogenous phospholipids | Avena sativa | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.2.1 | Cu2+ | - |
Avena sativa | |
| 7.1.2.1 | Cu2+ | - |
Saccharomyces cerevisiae | |
| 7.1.2.1 | Dicyclohexylcarbodiimide | - |
Avena sativa | |
| 7.1.2.1 | Dicyclohexylcarbodiimide | - |
Saccharomyces cerevisiae | |
| 7.1.2.1 | distilbestrol | - |
Avena sativa | |
| 7.1.2.1 | distilbestrol | - |
Saccharomyces cerevisiae | |
| 7.1.2.1 | vanadate | - |
Avena sativa | |
| 7.1.2.1 | vanadate | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.1.2.1 | 0.3 | - |
ATP | - |
Avena sativa | |
| 7.1.2.1 | 1 | - |
ATP | - |
Saccharomyces cerevisiae | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.1.2.1 | plasma membrane | - |
Saccharomyces cerevisiae | 5886 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.2.1 | Co2+ | can substitute for the physiological cofactor Mg2+ | Saccharomyces cerevisiae | |
| 7.1.2.1 | Co2+ | can substitute for the physiological cofactor Mg2+ | Avena sativa | |
| 7.1.2.1 | K+ | small activation | Saccharomyces cerevisiae | |
| 7.1.2.1 | K+ | small activation | Avena sativa | |
| 7.1.2.1 | Mg2+ | required | Saccharomyces cerevisiae | |
| 7.1.2.1 | Mg2+ | required | Avena sativa | |
| 7.1.2.1 | Mn2+ | can substitute for the physiological cofactor Mg2+ | Saccharomyces cerevisiae | |
| 7.1.2.1 | Mn2+ | can substitute for the physiological cofactor Mg2+ | Avena sativa | |
| 7.1.2.1 | Rb+ | small activation | Saccharomyces cerevisiae | |
| 7.1.2.1 | Rb+ | small activation | Avena sativa | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.1.2.1 | Avena sativa | - |
- |
- |
| 7.1.2.1 | Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.1.2.1 | - |
Avena sativa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 7.1.2.1 | root | - |
Avena sativa | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 7.1.2.1 | additional information | - |
- |
Saccharomyces cerevisiae |
| 7.1.2.1 | additional information | - |
- |
Avena sativa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.2.1 | ATP + H2O + H+/in | high substrate specificity for ATP | Saccharomyces cerevisiae | ADP + phosphate + H+/out | - |
? | |
| 7.1.2.1 | ATP + H2O + H+/in | high substrate specificity for ATP | Avena sativa | ADP + phosphate + H+/out | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.1.2.1 | 5.7 | - |
- |
Saccharomyces cerevisiae |
| 7.1.2.1 | 6.5 | - |
- |
Avena sativa |
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