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Literature summary extracted from

  • Okkeri, J.; Haltia, R.
    Expression and mutagenesis of ZntA, a zinc-transporting P-type ATPase from Escherichia coli (1999), Biochemistry, 38, 14109-14116.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
7.2.2.12 E470A metal ion-stimulated activity is reduced, about 30-40%. Mutant is phosphorylated more strongly in comparison to the wild type enzyme Escherichia coli
7.2.2.12 H475Q metal ion-stimulated activity is reduced, about 30-40%. Mutant is phosphorylated more weakly in comparison to the wild type enzyme. Mutation affects the reaction with ATP and phosphate and stabilizes the enzyme in a dephosphorylated state Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.2.2.12 Cu2+ prevents Zn2+ activation when present in 2fold excess over Zn2+ Escherichia coli
7.2.2.12 vanadate
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.2.12 membrane
-
 Escherichia coli 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.2.2.12 Ag+ activates ATPase activity Escherichia coli
7.2.2.12 Cu2+ enhances phosphorylation, it cannot activate the ATPase Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.2.2.12 92000
-
 1 * 92000, also detection of a dimeric form of 190000 Da, SDS-PAGE Escherichia coli
7.2.2.12 92000
-
 2 * 92000, also detection of a monomeric form of 92000 Da, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.12 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.2.12 ATP + H2O + Ag+/in
-
 Escherichia coli ADP + phosphate + Ag+/out
-
 ?
7.2.2.12 ATP + H2O + Cd2+/in
-
 Escherichia coli ADP + phosphate + Cd2+/out
-
 ?
7.2.2.12 ATP + H2O + Pb2+/in
-
 Escherichia coli ADP + phosphate + Pb2+/out
-
 ?
7.2.2.12 ATP + H2O + Zn2+/in
-
 Escherichia coli ADP + phosphate + Zn2+/out
-
 ?

Subunits

EC Number Subunits Comment Organism
7.2.2.12 dimer 2 * 92000, also detection of a monomeric form of 92000 Da, SDS-PAGE Escherichia coli
7.2.2.12 monomer 1 * 92000, also detection of a dimeric form of 190000 Da, SDS-PAGE Escherichia coli
7.2.2.12 More
-
 Escherichia coli