BRENDA - Enzyme Database

Purification and characterization of HisP, the ATP-binding subunit of a traffic ATPase (ABC transporter), the histidine permease of Salmonella typhimurium

Nikaido, K.; Liu, P.Q.; Ferro-Luzzi Ames, G.; J. Biol. Chem. 272, 27745-27752 (1997)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
7.4.2.1
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids is stable to freezing and thawing
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
preparation diluted to 0.7 mg/ml protein and 4% glycerol is stable at 0░C for at least 1 h
Salmonella enterica subsp. enterica serovar Typhimurium
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
7.4.2.1
2'-O-(trinitrophenyl)adenosine 5'-triphosphate
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
3'-O-(trinitrophenyl)adenosine 5'-triphosphate
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
adenosine 5'-O-(3-thio)triphosphate
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
adenylyl (beta,gamma-methylene)-diphosphate
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
adenylyl (beta,gamma-methylene)-diphosphonate
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
ADP
dimeric HisP protein with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
cardiolipin
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
lysophosphatidic acid
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
NEM
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
phosphatidic acid
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
phosphatidylglycerol
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
phosphatidylserine
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
vanadate
in contrast to the intact enzyme complex HisQMP2 the ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids - Leu-Glu-His-His-His-His-His-His is not inhibited
Salmonella enterica subsp. enterica serovar Typhimurium
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
7.4.2.1
Ca2+
poor stimulatory activity on ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
Co2+
dimeric ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids - Leu-Glu-His-His-His-His-His-His - under optimal conditions hydrolyzes ATP at a rate comparable to that of the intact complex HisQMP2, requires a cation for activity, Co2+ is the best stimulator at 1 mM
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
Mg2+
dimeric ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids - Leu-Glu-His-His-His-His-His-His - under optimal conditions hydrolyzes ATP at a rate comparable to that of the intact complex HisQMP2, requires a cation for activity, Co2+ and Mn2+ are more effective than Mg2+
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
Mn2+
dimeric ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids - Leu-Glu-His-His-His-His-His-His - under optimal conditions hydrolyzes ATP at a rate comparable to that of the intact complex HisQMP2, requires a cation for activity, Mn2+ is the best stimulator at concentrations above 1.5 mM
Salmonella enterica subsp. enterica serovar Typhimurium
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.4.2.1
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
7.4.2.1
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids, addition of a 6-histidine extension allows rapid and effective metal affinity purification; nucleotide-binding subunit HisP
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
7.4.2.1
additional information
-
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
Storage Stability
EC Number
Storage Stability
Organism
7.4.2.1
5-10 mg/ml in storage buffer and in liquid nitrogen, ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids is stable for at least several months
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.4.2.1
ATP + H2O + His/out
-
246888
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + phosphate + His/in
-
246888
Salmonella enterica subsp. enterica serovar Typhimurium
?
7.4.2.1
CTP + H2O + His/out
dimeric HisP protein with a carboxy-terminal extension of 8 amino acids, 50% of the ATPase activity with ATP
246888
Salmonella enterica subsp. enterica serovar Typhimurium
CDP + phosphate + His/in
-
246888
Salmonella enterica subsp. enterica serovar Typhimurium
?
7.4.2.1
GTP + H2O + His/out
dimeric HisP protein with a carboxy-terminal extension of 8 amino acids, 53% of the ATPase activity with ATP
246888
Salmonella enterica subsp. enterica serovar Typhimurium
GDP + phosphate + His/in
-
246888
Salmonella enterica subsp. enterica serovar Typhimurium
?
7.4.2.1
UTP + H2O + His/out
dimeric HisP protein with a carboxy-terminal extension of 8 amino acids, 37% of the ATPase activity with ATP
246888
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + phosphate + His/in
-
246888
Salmonella enterica subsp. enterica serovar Typhimurium
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4.2.1
7
-
dimeric HisP protein with a carboxy-terminal extension of 8 amino acids - Leu-Glu-His-His-His-His-His-His - under optimal conditions hydrolyzes ATP to a rate comparable to that by the intact complex HisQMP2
Salmonella enterica subsp. enterica serovar Typhimurium
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
7.4.2.1
6.3
8
pH 6.3: about 30% of maximal activity, pH 8.0: about 50% of maximal activity, dimeric HisP protein with a carboxy-terminal extension of 8 amino acids, Leu-Glu-His-His-His-His-His-His
Salmonella enterica subsp. enterica serovar Typhimurium
General Stability (protein specific)
EC Number
General Stability
Organism
7.4.2.1
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids is stable to freezing and thawing
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
preparation diluted to 0.7 mg/ml protein and 4% glycerol is stable at 0░C for at least 1 h
Salmonella enterica subsp. enterica serovar Typhimurium
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
7.4.2.1
2'-O-(trinitrophenyl)adenosine 5'-triphosphate
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
3'-O-(trinitrophenyl)adenosine 5'-triphosphate
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
adenosine 5'-O-(3-thio)triphosphate
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
adenylyl (beta,gamma-methylene)-diphosphate
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
adenylyl (beta,gamma-methylene)-diphosphonate
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
ADP
dimeric HisP protein with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
cardiolipin
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
lysophosphatidic acid
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
NEM
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
phosphatidic acid
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
phosphatidylglycerol
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
phosphatidylserine
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
vanadate
in contrast to the intact enzyme complex HisQMP2 the ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids - Leu-Glu-His-His-His-His-His-His is not inhibited
Salmonella enterica subsp. enterica serovar Typhimurium
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
7.4.2.1
Ca2+
poor stimulatory activity on ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
Co2+
dimeric ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids - Leu-Glu-His-His-His-His-His-His - under optimal conditions hydrolyzes ATP at a rate comparable to that of the intact complex HisQMP2, requires a cation for activity, Co2+ is the best stimulator at 1 mM
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
Mg2+
dimeric ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids - Leu-Glu-His-His-His-His-His-His - under optimal conditions hydrolyzes ATP at a rate comparable to that of the intact complex HisQMP2, requires a cation for activity, Co2+ and Mn2+ are more effective than Mg2+
Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.1
Mn2+
dimeric ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids - Leu-Glu-His-His-His-His-His-His - under optimal conditions hydrolyzes ATP at a rate comparable to that of the intact complex HisQMP2, requires a cation for activity, Mn2+ is the best stimulator at concentrations above 1.5 mM
Salmonella enterica subsp. enterica serovar Typhimurium
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
7.4.2.1
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids, addition of a 6-histidine extension allows rapid and effective metal affinity purification; nucleotide-binding subunit HisP
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
7.4.2.1
additional information
-
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
Salmonella enterica subsp. enterica serovar Typhimurium
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
7.4.2.1
5-10 mg/ml in storage buffer and in liquid nitrogen, ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids is stable for at least several months
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.4.2.1
ATP + H2O + His/out
-
246888
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + phosphate + His/in
-
246888
Salmonella enterica subsp. enterica serovar Typhimurium
?
7.4.2.1
CTP + H2O + His/out
dimeric HisP protein with a carboxy-terminal extension of 8 amino acids, 50% of the ATPase activity with ATP
246888
Salmonella enterica subsp. enterica serovar Typhimurium
CDP + phosphate + His/in
-
246888
Salmonella enterica subsp. enterica serovar Typhimurium
?
7.4.2.1
GTP + H2O + His/out
dimeric HisP protein with a carboxy-terminal extension of 8 amino acids, 53% of the ATPase activity with ATP
246888
Salmonella enterica subsp. enterica serovar Typhimurium
GDP + phosphate + His/in
-
246888
Salmonella enterica subsp. enterica serovar Typhimurium
?
7.4.2.1
UTP + H2O + His/out
dimeric HisP protein with a carboxy-terminal extension of 8 amino acids, 37% of the ATPase activity with ATP
246888
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + phosphate + His/in
-
246888
Salmonella enterica subsp. enterica serovar Typhimurium
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4.2.1
7
-
dimeric HisP protein with a carboxy-terminal extension of 8 amino acids - Leu-Glu-His-His-His-His-His-His - under optimal conditions hydrolyzes ATP to a rate comparable to that by the intact complex HisQMP2
Salmonella enterica subsp. enterica serovar Typhimurium
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
7.4.2.1
6.3
8
pH 6.3: about 30% of maximal activity, pH 8.0: about 50% of maximal activity, dimeric HisP protein with a carboxy-terminal extension of 8 amino acids, Leu-Glu-His-His-His-His-His-His
Salmonella enterica subsp. enterica serovar Typhimurium