Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Daleke, D.L.; Lyles, J.V.
    Identification and purification of aminophospholipid flippases (2000), Biochim. Biophys. Acta, 1486, 108-127.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.6.2.1 dithiothreitol partially stimulates Homo sapiens
7.6.2.1 F-
-
 Homo sapiens
7.6.2.1 glycerophosphoserine prevents transport of phosphatidylserine Homo sapiens
7.6.2.1 GPS
-
 Homo sapiens
7.6.2.1 phenylacylbromide
-
 Homo sapiens
7.6.2.1 Phenylglyoxal
-
 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.6.2.1 Ca2+ 0.0002 mM, inhibits from the cytofacial surface Homo sapiens
7.6.2.1 NEM
-
 Homo sapiens
7.6.2.1 vanadate
-
 Bos taurus
7.6.2.1 vanadate inhibits the enzyme from the cytofacial surface Homo sapiens
7.6.2.1 vanadyl ion inhibits the enzyme from the extracellular surface Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.6.2.1 plasma membrane plasma membrane Homo sapiens 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.6.2.1 Mg2+ required Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.6.2.1 110000
-
 x * 110000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.6.2.1 ATP + H2O Bos taurus
-
 ADP + phosphate
-
 ?
7.6.2.1 ATP + H2O Homo sapiens role in aminophospholipid transport ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.6.2.1 Bos taurus
-
-
-
7.6.2.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.6.2.1 partial Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.6.2.1 aorta epithelial cells Bos taurus
-
7.6.2.1 brain
-
 Bos taurus
-
7.6.2.1 brain synaptosomes Homo sapiens
-
7.6.2.1 epithelial cell
-
 Homo sapiens
-
7.6.2.1 erythrocyte
-
 Homo sapiens
-
7.6.2.1 fibroblast
-
 Homo sapiens
-
7.6.2.1 hepatocyte
-
 Homo sapiens
-
7.6.2.1 lymphocyte
-
 Homo sapiens
-
7.6.2.1 pheochromocytoma cell
-
 Homo sapiens
-
7.6.2.1 spermatozoon
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.6.2.1 ATP + H2O
-
 Bos taurus ADP + phosphate
-
 ?
7.6.2.1 ATP + H2O transport of ethanolamine at a rate 10fold slower than phosphatidylserine Homo sapiens ADP + phosphate
-
 ?
7.6.2.1 ATP + H2O transport of phosphatidylserine Homo sapiens ADP + phosphate
-
 ?
7.6.2.1 ATP + H2O the protein serves primarily to prevent the accumulation of phosphatidylserine on the outer surface of the cell Homo sapiens ADP + phosphate
-
 ?
7.6.2.1 ATP + H2O transport of N-methylphosphatidylserine Homo sapiens ADP + phosphate
-
 ?
7.6.2.1 ATP + H2O role in aminophospholipid transport Homo sapiens ADP + phosphate
-
 ?
7.6.2.1 dATP + H2O
-
 Homo sapiens dADP + H2O
-
 ?
7.6.2.1 additional information flippase recognizes multiple structural elements in its preferred lipid substrate. The lipid structural elements include a primary amine group, limited headgroup size and and sn-1,2-glycerol backbone. The presence of carboxyl group accelerates transport, but is not required for activity Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
7.6.2.1 ? x * 110000, SDS-PAGE Homo sapiens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
7.6.2.1 additional information
-
 below 4°C transport of phospholipids is inhibited in both erythrocytes and fibroblasts, warming to 7°C activates transport in fibroblasts Homo sapiens