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Literature summary extracted from

  • Decker, K.; Bischoff, E.
    Purification and properties of nucleotide pyrophosphatase from rat liver plasma membranes (1972), FEBS Lett., 21, 95-98.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.9 alpha,beta-methylene adenosine 5'-triphosphate competitive inhibition, Ki: 25 mM Rattus sp.
3.6.1.9 AMP-amidate competitive inhibition, Ki: 30 mM Rattus sp.
3.6.1.9 EDTA complete inactivation, partially restored by Mg2+, Ca2+ Rattus sp.
3.6.1.9 UDP-glucose competitive inhibition, Ki: 18 mM Rattus sp.
3.6.1.9 UMP competitive inhibition, Ki: 26 mM Rattus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.9 20
-
 thymidine 5'-monophosphate p-nitrophenyl ester
-
 Rattus sp.
3.6.1.9 33
-
 UTP
-
 Rattus sp.
3.6.1.9 50
-
 UDP-glucose
-
 Rattus sp.
3.6.1.9 78
-
 ATP
-
 Rattus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.9 plasma membrane
-
 Rattus sp. 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.9 Ca2+ required for optimal activity Rattus sp.
3.6.1.9 Mg2+ required for optimal activity Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.9 ADP + H2O Rattus sp. low activity AMP + phosphate
-
 ?
3.6.1.9 ATP + H2O Rattus sp.
-
 AMP + diphosphate
-
 ?
3.6.1.9 DNA + H2O Rattus sp. E. coli, low activity adenosine 5'-monophosphate + ?
-
 ?
3.6.1.9 polyadenylate + H2O Rattus sp. low activity adenosine 5'-monophosphate + ?
-
 ?
3.6.1.9 RNA + H2O Rattus sp. yeast, low activity adenosine 5'-monophosphate + ?
-
 ?
3.6.1.9 UDP + H2O Rattus sp.
-
 UMP + phosphate low activity ?
3.6.1.9 UDP-glucose + H2O Rattus sp.
-
 UMP + D-glucose-1-phosphate
-
 ?
3.6.1.9 UTP + H2O Rattus sp.
-
 UMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.9 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.9 to homogeneity, chromatography rechniques Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.9 liver
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.9 151
-
-
 Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.9 ADP + H2O low activity Rattus sp. AMP + phosphate
-
 ?
3.6.1.9 ATP + H2O
-
 Rattus sp. AMP + diphosphate
-
 ?
3.6.1.9 DNA + H2O E. coli, low activity Rattus sp. adenosine 5'-monophosphate + ?
-
 ?
3.6.1.9 polyadenylate + H2O low activity Rattus sp. adenosine 5'-monophosphate + ?
-
 ?
3.6.1.9 RNA + H2O yeast, low activity Rattus sp. adenosine 5'-monophosphate + ?
-
 ?
3.6.1.9 thymidine 5'-monophosphate p-nitrophenyl ester + H2O significant phosphodiesterase activity Rattus sp. thymidine 5'-monophosphate + p-nitrophenol
-
 ?
3.6.1.9 UDP + H2O
-
 Rattus sp. UMP + phosphate low activity ?
3.6.1.9 UDP-glucose + H2O
-
 Rattus sp. UMP + D-glucose-1-phosphate
-
 ?
3.6.1.9 UTP + H2O
-
 Rattus sp. UMP + diphosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.9 9
-
-
 Rattus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.6.1.9 6
-
 instability below Rattus sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.9 25
-
 alpha,beta-methylene adenosine 5'-triphosphate
-
 Rattus sp.