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Literature summary extracted from

  • Balakrishnan, C.V.; Ravindranath, S.D.; Appaji Rao, N.
    Studies on nucleotidases in plants. Reversible denaturation of the crystalline mung bean nucleotide pyrophosphatase and the effect of adenylates on the native and renatured enzyme (1974), Arch. Biochem. Biophys., 164, 156-164.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.9 5'-ADP less potent inhibitor Vigna radiata var. radiata
3.6.1.9 5'-AMP biphasic inhibition, increasing Km, decreasing Vm Vigna radiata var. radiata
3.6.1.9 ATP non-competitive inhibition Vigna radiata var. radiata
3.6.1.9 EDTA reversal by Zn2+ Vigna radiata var. radiata
3.6.1.9 guanidine hydrochloride 85% inhibition at 4.2 M, complete recovery of activity after dialysis Vigna radiata var. radiata
3.6.1.9 SDS partial inactivation at 0.1%, w/v, complete inactivation at 1%, w/v Vigna radiata var. radiata
3.6.1.9 Urea progressive inhibition greater 1 M, 80% inhibition at 6 M, dilution or dialysis results in complete restoration of activity Vigna radiata var. radiata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.9 0.2
-
 FAD
-
 Vigna radiata var. radiata
3.6.1.9 0.3
-
 NAD+
-
 Vigna radiata var. radiata
3.6.1.9 1.8
-
 FAD after urea-denaturation-renaturation Vigna radiata var. radiata
3.6.1.9 2.2
-
 NAD+ after urea-denaturation-renaturation Vigna radiata var. radiata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.9 FAD + H2O Vigna radiata var. radiata
-
 FMN + AMP
-
 ?
3.6.1.9 NAD+ + H2O Vigna radiata var. radiata
-
 NMN + AMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.9 Vigna radiata var. radiata
-
 mung bean
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.9 FAD + H2O
-
 Vigna radiata var. radiata FMN + AMP
-
 ?
3.6.1.9 NAD+ + H2O
-
 Vigna radiata var. radiata NMN + AMP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.9 49
-
-
 Vigna radiata var. radiata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.9 9.4
-
-
 Vigna radiata var. radiata