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Literature summary extracted from

  • Balakrishnan, C.V.; Vaidyanathan, C.S.; Rao, N.A.
    Studies on nucleotidases in plants. Isolation and properties of the monomeric form of the crystalline and homogeneous mung bean nucleotide pyrophosphatase (1977), Eur. J. Biochem., 78, 95-102.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.9 5'-ADP inhibition of monomer Vigna radiata var. radiata
3.6.1.9 5'-AMP complete inhibition at 1 mM for monomer, dimer, tetramer, only dimer inhibits at 0.01 mM to 25% Vigna radiata var. radiata
3.6.1.9 ATP inhibition of monomer Vigna radiata var. radiata
3.6.1.9 EDTA complete inhibition, reversal for dimer and tetramer by Zn2+ Vigna radiata var. radiata
3.6.1.9 Urea complete inhibition at 4.5 M for monomer, tetramer inactivated at 6 M Vigna radiata var. radiata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.9 0.25
-
 FAD value for dimeric form, biphasic Vigna radiata var. radiata
3.6.1.9 0.5
-
 FAD value for monomeric form Vigna radiata var. radiata
3.6.1.9 0.58
-
 FAD value for tetrameric form Vigna radiata var. radiata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.9 33000
-
 1 * 33000, p-hydroxymercuribenzoate treated preparation leads to dissociation to an active monomer, SDS-PAGE Vigna radiata var. radiata
3.6.1.9 33000
-
 2 * 33000, SDS-PAGE, addition of AMP converts dimer to an active tetramer Vigna radiata var. radiata
3.6.1.9 66000
-
 gel filtration Vigna radiata var. radiata
3.6.1.9 70000
-
 gel filtration Vigna radiata var. radiata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.9 FAD + H2O Vigna radiata var. radiata
-
 FMN + AMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.9 Vigna radiata var. radiata
-
 mung bean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.9 to homogeneity, enzymatically active monomer, dimer and tetramer Vigna radiata var. radiata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.9 FAD + H2O
-
 Vigna radiata var. radiata FMN + AMP
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.9 dimer 2 * 33000, SDS-PAGE, addition of AMP converts dimer to an active tetramer Vigna radiata var. radiata
3.6.1.9 monomer 1 * 33000, p-hydroxymercuribenzoate treated preparation leads to dissociation to an active monomer, SDS-PAGE Vigna radiata var. radiata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.9 37
-
 monomeric form Vigna radiata var. radiata
3.6.1.9 49
-
 dimeric, tetrameric forms Vigna radiata var. radiata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.9 55
-
 half-life: monomer 3 min, dimer 20 min, tetramer 15 min Vigna radiata var. radiata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.9 9.4
-
-
 Vigna radiata var. radiata