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Literature summary extracted from

  • Twu, J.S.; Haroz, R.K.; Bretthauer, R.K.
    Nucleotide pyrophosphatase from yeast. The presence of bound zinc (1977), Arch. Biochem. Biophys., 184, 249-256.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.9 2-mercaptoethanol 19% inhibition at 4 mM Saccharomyces cerevisiae
3.6.1.9 dithiothreitol 92% inhibition at 4 mM, reversal to 92% of initial activity by addition of Zn2+ Saccharomyces cerevisiae
3.6.1.9 EDTA complete inactivation at 2 mM, no reversal by addition of metals Saccharomyces cerevisiae
3.6.1.9 L-ascorbic acid 8% inhibition at 16 mM Saccharomyces cerevisiae
3.6.1.9 L-cysteine complete inhibition at 4 mM, reversal to 95% of initial activity by addition of Zn2+ Saccharomyces cerevisiae
3.6.1.9 o-phenanthroline complete inactivation at 2 mM, no reversal by addition of metal ions, m-phenanthroline no effect Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.9 Zn2+ tightly bound, contains 1 zinc atom per mol of enzyme Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.9 GDP-mannose + H2O Saccharomyces cerevisiae
-
GMP + D-mannose-1-phosphate
-
?
3.6.1.9 UDP-glucose + H2O Saccharomyces cerevisiae
-
UMP + D-glucose-1-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.9 Saccharomyces cerevisiae
-
baker's yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.9 chromatography techniques Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.9 GDP-mannose + H2O
-
Saccharomyces cerevisiae GMP + D-mannose-1-phosphate
-
?
3.6.1.9 p-nitrophenyl-dTMP + H2O
-
Saccharomyces cerevisiae p-nitrophenol + dTMP
-
?
3.6.1.9 UDP-glucose + H2O
-
Saccharomyces cerevisiae UMP + D-glucose-1-phosphate
-
?