BRENDA - Enzyme Database

Nucleotide pyrophosphatase from potato tubers. Purification and properties

Bartkiewicz, M.; Sierakowska, H.; Shugar, D.; Eur. J. Biochem. 143, 419-426 (1984)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.9
2',3'-cAMP
7% inhibition at 2 mM
Solanum tuberosum
3.6.1.9
3',5'-cAMP
4% inhibition at 2 mM
Solanum tuberosum
3.6.1.9
5'-AMP
56% inhibition at 2 mM
Solanum tuberosum
3.6.1.9
adenosine 2'-monophosphate
8% inhibition at 2 mM
Solanum tuberosum
3.6.1.9
adenosine 3'-monophosphate
8% inhibition at 2 mM
Solanum tuberosum
3.6.1.9
alpha,beta-methylene adenosine 5'-triphosphate
25% inhibition at 2 mM
Solanum tuberosum
3.6.1.9
ATP
44% inhibition at 2 mM
Solanum tuberosum
3.6.1.9
diphosphate
8% inhibition at 2 mM
Solanum tuberosum
3.6.1.9
NAD+
84% inhibition at 2 mM
Solanum tuberosum
3.6.1.9
phosphate
18% inhibition at 2 mM
Solanum tuberosum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.9
0.05
-
5',5'-diadenosine diphosphate
-
Solanum tuberosum
3.6.1.9
0.05
-
5',5'-diadenosine triphosphate
-
Solanum tuberosum
3.6.1.9
0.05
-
NAD+
-
Solanum tuberosum
3.6.1.9
0.1
-
7-methyl 5'-guanosine 2'-O-methylguanosine triphosphate
-
Solanum tuberosum
3.6.1.9
0.2
-
5',5'-diadenosine tetraphosphate
-
Solanum tuberosum
3.6.1.9
0.5
-
ATP
-
Solanum tuberosum
3.6.1.9
0.5
-
dATP
-
Solanum tuberosum
3.6.1.9
1
-
thymidine 5'-monophosphate p-nitrophenyl ester
-
Solanum tuberosum
3.6.1.9
1.3
-
thymidine 5'-diphospho 5'-thymidine
-
Solanum tuberosum
3.6.1.9
2.3
-
2',3'-cAMP
-
Solanum tuberosum
3.6.1.9
2.7
-
thymidine 3'-diphospho 3'-thymidine
-
Solanum tuberosum
3.6.1.9
6.1
-
thymidine 3'-monophosphate p-nitrophenyl ester
-
Solanum tuberosum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.9
74000
-
4 * 74000, SDS-PAGE
Solanum tuberosum
3.6.1.9
346000
-
sucrose density gradient centrifugation
Solanum tuberosum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.9
2',3'-cAMP + H2O
Solanum tuberosum
low activity
adenosine monophosphate
-
Solanum tuberosum
?
3.6.1.9
3',5'-cAMP + H2O
Solanum tuberosum
traces of activity
adenosine monophosphate
-
Solanum tuberosum
?
3.6.1.9
5'-AMP + H2O
Solanum tuberosum
traces of activity
adenosine + phosphate
-
Solanum tuberosum
?
3.6.1.9
ADP + H2O
Solanum tuberosum
low activity
AMP + phosphate
-
Solanum tuberosum
?
3.6.1.9
ATP + H2O
Solanum tuberosum
-
AMP + diphosphate
initial product: AMP
Solanum tuberosum
?
3.6.1.9
CDP + H2O
Solanum tuberosum
low activity
CMP + phosphate
-
Solanum tuberosum
?
3.6.1.9
CTP + H2O
Solanum tuberosum
low activity
?
-
Solanum tuberosum
?
3.6.1.9
dATP + H2O
Solanum tuberosum
-
?
-
Solanum tuberosum
?
3.6.1.9
mRNA + H2O
Solanum tuberosum
reovirus m7-G(5')p3(5')Gm-terminated intact mRNA
7-methyl-GMP + ?
-
Solanum tuberosum
?
3.6.1.9
NAD+ + H2O
Solanum tuberosum
-
NMN + AMP
-
Solanum tuberosum
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.9
Solanum tuberosum
-
potato
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.6.1.9
glycoprotein
-
Solanum tuberosum
Purification (Commentary)
EC Number
Commentary
Organism
3.6.1.9
to homogeneity, chromatography techniques
Solanum tuberosum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.6.1.9
tuber
-
Solanum tuberosum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.6.1.9
2831
-
-
Solanum tuberosum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.9
2',3'-cAMP + H2O
low activity
246817
Solanum tuberosum
adenosine monophosphate
-
246817
Solanum tuberosum
?
3.6.1.9
3',5'-cAMP + H2O
traces of activity
246817
Solanum tuberosum
adenosine monophosphate
-
246817
Solanum tuberosum
?
3.6.1.9
5'-AMP + H2O
traces of activity
246817
Solanum tuberosum
adenosine + phosphate
-
246817
Solanum tuberosum
?
3.6.1.9
7-methyl 5'-guanosine 2'-O-methylgunaosine triphosphate + H2O
-
246817
Solanum tuberosum
7-methyl-GMP + 7-methyl-GDP + GMP + GDP
-
246817
Solanum tuberosum
?
3.6.1.9
8-bromo-NAD+ + H2O
same hydrolysis rate as for NAD+
246817
Solanum tuberosum
8-bromo-AMP + NMN
-
246817
Solanum tuberosum
?
3.6.1.9
adenosine (alpha,beta-methylene) 5'-triphosphate + H2O
-
246817
Solanum tuberosum
adenosine (alpha,beta-methylene) 5'-diphosphate + phosphate
-
246817
Solanum tuberosum
?
3.6.1.9
ADP + H2O
low activity
246817
Solanum tuberosum
AMP + phosphate
-
246817
Solanum tuberosum
?
3.6.1.9
ATP + H2O
-
246817
Solanum tuberosum
AMP + diphosphate
initial product: AMP
246817
Solanum tuberosum
?
3.6.1.9
beta-naphthylphenylphosphonate + H2O
very low activity
246817
Solanum tuberosum
?
-
246817
Solanum tuberosum
?
3.6.1.9
bis(p-nitrophenyl)phosphate + H2O
-
246817
Solanum tuberosum
p-nitrophenol + p-nitrophenylphosphate
-
246817
Solanum tuberosum
?
3.6.1.9
CDP + H2O
low activity
246817
Solanum tuberosum
CMP + phosphate
-
246817
Solanum tuberosum
?
3.6.1.9
CTP + H2O
low activity
246817
Solanum tuberosum
?
-
246817
Solanum tuberosum
?
3.6.1.9
dATP + H2O
-
246817
Solanum tuberosum
?
-
246817
Solanum tuberosum
?
3.6.1.9
diadenosine 5'-diphosphate + H2O
-
246817
Solanum tuberosum
adenosine 5'-monophosphate + ?
-
-
-
-
3.6.1.9
diadenosine polyphosphate + H2O
from di- to pentaphosphates, hydrolysis rate decreases with chain length
246817
Solanum tuberosum
AMP + ?
-
246817
Solanum tuberosum
?
3.6.1.9
guanosine (alpha,beta-methylene) 5'-triphosphate + H2O
-
246817
Solanum tuberosum
guanosine (alpha,beta-methylene) 5'-diphosphate + phosphate
-
246817
Solanum tuberosum
?
3.6.1.9
mRNA + H2O
reovirus m7-G(5')p3(5')Gm-terminated intact mRNA
246817
Solanum tuberosum
7-methyl-GMP + ?
-
246817
Solanum tuberosum
?
3.6.1.9
NAD+ + H2O
-
246817
Solanum tuberosum
NMN + AMP
-
246817
Solanum tuberosum
?
3.6.1.9
p-nitrophenyl phenylphosphonate + H2O
low activity
246817
Solanum tuberosum
p-nitrophenol + phenylphosphonate
-
246817
Solanum tuberosum
?
3.6.1.9
p-nitrophenylphosphate + H2O
-
246817
Solanum tuberosum
p-nitrophenol + phosphate
-
246817
Solanum tuberosum
?
3.6.1.9
thymidine 3'-diphospho 3'-thymidine + H2O
very low activity
246817
Solanum tuberosum
thymidine 3'-phosphate + thymidine 3'-phosphate
-
246817
Solanum tuberosum
?
3.6.1.9
thymidine 3'-monophosphate p-nitrophenyl ester + H2O
low activity
246817
Solanum tuberosum
thymidine 3'-monophosphate + p-nitrophenol
-
246817
Solanum tuberosum
?
3.6.1.9
thymidine 5'-6-bromo-2-hydroxy-3-naphthoic acid 2-methoxyanilide-naphthylphosphate + H2O
low activity
246817
Solanum tuberosum
?
-
246817
Solanum tuberosum
?
3.6.1.9
thymidine 5'-alpha-naphthylphosphate + H2O
very low activity
246817
Solanum tuberosum
thymidine + alpha-naphthylphosphate
-
246817
Solanum tuberosum
?
3.6.1.9
thymidine 5'-diphospho 5'-thymidine + H2O
low activity
246817
Solanum tuberosum
thymidine 5'-phosphate + thymidine 5'-phosphate
-
246817
Solanum tuberosum
?
3.6.1.9
thymidine 5'-monophosphate p-nitrophenyl ester + H2O
-
246817
Solanum tuberosum
thymidine 5'-monophosphate + p-nitrophenol
-
246817
Solanum tuberosum
?
Subunits
EC Number
Subunits
Commentary
Organism
3.6.1.9
tetramer
4 * 74000, SDS-PAGE
Solanum tuberosum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.9
2',3'-cAMP
7% inhibition at 2 mM
Solanum tuberosum
3.6.1.9
3',5'-cAMP
4% inhibition at 2 mM
Solanum tuberosum
3.6.1.9
5'-AMP
56% inhibition at 2 mM
Solanum tuberosum
3.6.1.9
adenosine 2'-monophosphate
8% inhibition at 2 mM
Solanum tuberosum
3.6.1.9
adenosine 3'-monophosphate
8% inhibition at 2 mM
Solanum tuberosum
3.6.1.9
alpha,beta-methylene adenosine 5'-triphosphate
25% inhibition at 2 mM
Solanum tuberosum
3.6.1.9
ATP
44% inhibition at 2 mM
Solanum tuberosum
3.6.1.9
diphosphate
8% inhibition at 2 mM
Solanum tuberosum
3.6.1.9
NAD+
84% inhibition at 2 mM
Solanum tuberosum
3.6.1.9
phosphate
18% inhibition at 2 mM
Solanum tuberosum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.9
0.05
-
5',5'-diadenosine diphosphate
-
Solanum tuberosum
3.6.1.9
0.05
-
5',5'-diadenosine triphosphate
-
Solanum tuberosum
3.6.1.9
0.05
-
NAD+
-
Solanum tuberosum
3.6.1.9
0.1
-
7-methyl 5'-guanosine 2'-O-methylguanosine triphosphate
-
Solanum tuberosum
3.6.1.9
0.2
-
5',5'-diadenosine tetraphosphate
-
Solanum tuberosum
3.6.1.9
0.5
-
ATP
-
Solanum tuberosum
3.6.1.9
0.5
-
dATP
-
Solanum tuberosum
3.6.1.9
1
-
thymidine 5'-monophosphate p-nitrophenyl ester
-
Solanum tuberosum
3.6.1.9
1.3
-
thymidine 5'-diphospho 5'-thymidine
-
Solanum tuberosum
3.6.1.9
2.3
-
2',3'-cAMP
-
Solanum tuberosum
3.6.1.9
2.7
-
thymidine 3'-diphospho 3'-thymidine
-
Solanum tuberosum
3.6.1.9
6.1
-
thymidine 3'-monophosphate p-nitrophenyl ester
-
Solanum tuberosum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.9
74000
-
4 * 74000, SDS-PAGE
Solanum tuberosum
3.6.1.9
346000
-
sucrose density gradient centrifugation
Solanum tuberosum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.9
2',3'-cAMP + H2O
Solanum tuberosum
low activity
adenosine monophosphate
-
Solanum tuberosum
?
3.6.1.9
3',5'-cAMP + H2O
Solanum tuberosum
traces of activity
adenosine monophosphate
-
Solanum tuberosum
?
3.6.1.9
5'-AMP + H2O
Solanum tuberosum
traces of activity
adenosine + phosphate
-
Solanum tuberosum
?
3.6.1.9
ADP + H2O
Solanum tuberosum
low activity
AMP + phosphate
-
Solanum tuberosum
?
3.6.1.9
ATP + H2O
Solanum tuberosum
-
AMP + diphosphate
initial product: AMP
Solanum tuberosum
?
3.6.1.9
CDP + H2O
Solanum tuberosum
low activity
CMP + phosphate
-
Solanum tuberosum
?
3.6.1.9
CTP + H2O
Solanum tuberosum
low activity
?
-
Solanum tuberosum
?
3.6.1.9
dATP + H2O
Solanum tuberosum
-
?
-
Solanum tuberosum
?
3.6.1.9
mRNA + H2O
Solanum tuberosum
reovirus m7-G(5')p3(5')Gm-terminated intact mRNA
7-methyl-GMP + ?
-
Solanum tuberosum
?
3.6.1.9
NAD+ + H2O
Solanum tuberosum
-
NMN + AMP
-
Solanum tuberosum
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.6.1.9
glycoprotein
-
Solanum tuberosum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.9
to homogeneity, chromatography techniques
Solanum tuberosum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.6.1.9
tuber
-
Solanum tuberosum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.6.1.9
2831
-
-
Solanum tuberosum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.9
2',3'-cAMP + H2O
low activity
246817
Solanum tuberosum
adenosine monophosphate
-
246817
Solanum tuberosum
?
3.6.1.9
3',5'-cAMP + H2O
traces of activity
246817
Solanum tuberosum
adenosine monophosphate
-
246817
Solanum tuberosum
?
3.6.1.9
5'-AMP + H2O
traces of activity
246817
Solanum tuberosum
adenosine + phosphate
-
246817
Solanum tuberosum
?
3.6.1.9
7-methyl 5'-guanosine 2'-O-methylgunaosine triphosphate + H2O
-
246817
Solanum tuberosum
7-methyl-GMP + 7-methyl-GDP + GMP + GDP
-
246817
Solanum tuberosum
?
3.6.1.9
8-bromo-NAD+ + H2O
same hydrolysis rate as for NAD+
246817
Solanum tuberosum
8-bromo-AMP + NMN
-
246817
Solanum tuberosum
?
3.6.1.9
adenosine (alpha,beta-methylene) 5'-triphosphate + H2O
-
246817
Solanum tuberosum
adenosine (alpha,beta-methylene) 5'-diphosphate + phosphate
-
246817
Solanum tuberosum
?
3.6.1.9
ADP + H2O
low activity
246817
Solanum tuberosum
AMP + phosphate
-
246817
Solanum tuberosum
?
3.6.1.9
ATP + H2O
-
246817
Solanum tuberosum
AMP + diphosphate
initial product: AMP
246817
Solanum tuberosum
?
3.6.1.9
beta-naphthylphenylphosphonate + H2O
very low activity
246817
Solanum tuberosum
?
-
246817
Solanum tuberosum
?
3.6.1.9
bis(p-nitrophenyl)phosphate + H2O
-
246817
Solanum tuberosum
p-nitrophenol + p-nitrophenylphosphate
-
246817
Solanum tuberosum
?
3.6.1.9
CDP + H2O
low activity
246817
Solanum tuberosum
CMP + phosphate
-
246817
Solanum tuberosum
?
3.6.1.9
CTP + H2O
low activity
246817
Solanum tuberosum
?
-
246817
Solanum tuberosum
?
3.6.1.9
dATP + H2O
-
246817
Solanum tuberosum
?
-
246817
Solanum tuberosum
?
3.6.1.9
diadenosine 5'-diphosphate + H2O
-
246817
Solanum tuberosum
adenosine 5'-monophosphate + ?
-
-
-
-
3.6.1.9
diadenosine polyphosphate + H2O
from di- to pentaphosphates, hydrolysis rate decreases with chain length
246817
Solanum tuberosum
AMP + ?
-
246817
Solanum tuberosum
?
3.6.1.9
guanosine (alpha,beta-methylene) 5'-triphosphate + H2O
-
246817
Solanum tuberosum
guanosine (alpha,beta-methylene) 5'-diphosphate + phosphate
-
246817
Solanum tuberosum
?
3.6.1.9
mRNA + H2O
reovirus m7-G(5')p3(5')Gm-terminated intact mRNA
246817
Solanum tuberosum
7-methyl-GMP + ?
-
246817
Solanum tuberosum
?
3.6.1.9
NAD+ + H2O
-
246817
Solanum tuberosum
NMN + AMP
-
246817
Solanum tuberosum
?
3.6.1.9
p-nitrophenyl phenylphosphonate + H2O
low activity
246817
Solanum tuberosum
p-nitrophenol + phenylphosphonate
-
246817
Solanum tuberosum
?
3.6.1.9
p-nitrophenylphosphate + H2O
-
246817
Solanum tuberosum
p-nitrophenol + phosphate
-
246817
Solanum tuberosum
?
3.6.1.9
thymidine 3'-diphospho 3'-thymidine + H2O
very low activity
246817
Solanum tuberosum
thymidine 3'-phosphate + thymidine 3'-phosphate
-
246817
Solanum tuberosum
?
3.6.1.9
thymidine 3'-monophosphate p-nitrophenyl ester + H2O
low activity
246817
Solanum tuberosum
thymidine 3'-monophosphate + p-nitrophenol
-
246817
Solanum tuberosum
?
3.6.1.9
thymidine 5'-6-bromo-2-hydroxy-3-naphthoic acid 2-methoxyanilide-naphthylphosphate + H2O
low activity
246817
Solanum tuberosum
?
-
246817
Solanum tuberosum
?
3.6.1.9
thymidine 5'-alpha-naphthylphosphate + H2O
very low activity
246817
Solanum tuberosum
thymidine + alpha-naphthylphosphate
-
246817
Solanum tuberosum
?
3.6.1.9
thymidine 5'-diphospho 5'-thymidine + H2O
low activity
246817
Solanum tuberosum
thymidine 5'-phosphate + thymidine 5'-phosphate
-
246817
Solanum tuberosum
?
3.6.1.9
thymidine 5'-monophosphate p-nitrophenyl ester + H2O
-
246817
Solanum tuberosum
thymidine 5'-monophosphate + p-nitrophenol
-
246817
Solanum tuberosum
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.6.1.9
tetramer
4 * 74000, SDS-PAGE
Solanum tuberosum