BRENDA - Enzyme Database

Catabolism of bis(5-nucleosidyl) oligophosphates in Escherichia coli: metal requirements and substrate specificity of homogeneous diadenosine-5,5-P1,P4-tetraphosphate pyrophosphohydrolase

Plateau, P.; Fromant, M.; Brevet, A.; Gesquiere, A.; Blanquet, S.; Biochemistry 24, 914-922 (1985)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.41
Zn2+
exposure prior to the assay
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.41
0.012
-
P1,P4-bis(5'-adenosyl) tetraphosphate
with Mn2+
Escherichia coli
3.6.1.41
0.022
-
P1,P4-bis(5'-adenosyl) tetraphosphate
with Co2+
Escherichia coli
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.41
Ca2+
no effect
Escherichia coli
3.6.1.41
Co2+
2500fold stimulation
Escherichia coli
3.6.1.41
Mn2+
900fold stimulation
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.41
33000
-
gel filtration
Escherichia coli
3.6.1.41
34000
-
1 * 34000, SDS-PAGE
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.41
Escherichia coli
-
EM20031
-
3.6.1.41
Escherichia coli EM20031
-
EM20031
-
Purification (Commentary)
EC Number
Commentary
Organism
3.6.1.41
-
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.6.1.41
additional information
-
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.41
adenosine 5'-P(1)-tetraphosphate-P(4)-5'''-cytidine + H2O
-
246770
Escherichia coli
ADP + CDP
-
246770
Escherichia coli
?
3.6.1.41
adenosine 5'-P(1)-triphosphate-P(3)-5''-cytidine + H2O
-
246770
Escherichia coli
ADP + CMP + CDP + AMP
-
246770
Escherichia coli
?
3.6.1.41
adenosine 5'-P(1)-triphosphate-P(3)-5''-guanosine + H2O
-
246770
Escherichia coli
ADP + GMP
-
246770
Escherichia coli
?
3.6.1.41
Ap3A + H2O
-
246770
Escherichia coli
ADP + AMP
-
-
-
?
3.6.1.41
Ap3A + H2O
-
246770
Escherichia coli EM20031
ADP + AMP
-
-
-
?
3.6.1.41
Ap4 + H2O
-
246770
Escherichia coli
?
-
-
-
?
3.6.1.41
Ap4G + H2O
-
246770
Escherichia coli
ADP + GDP
-
246770
Escherichia coli
?
3.6.1.41
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
-
246770
Escherichia coli
ADP + ATP
-
246770
Escherichia coli
?
3.6.1.41
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
-
246770
Escherichia coli EM20031
ADP + ATP
-
246770
Escherichia coli EM20031
?
3.6.1.41
Gp3C + H2O
-
246770
Escherichia coli
CDP + GMP
-
246770
Escherichia coli
?
3.6.1.41
Gp3G + H2O
-
246770
Escherichia coli
guanosine 3'-diphosphate + guanosine 5'-phosphate
-
-
-
?
3.6.1.41
P1,P4-bis(5'-(2'-deoxy)adenosyl)tetraphosphate + H2O
-
246770
Escherichia coli
2 dADP
-
246770
Escherichia coli
?
3.6.1.41
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
246770
Escherichia coli
ADP
-
246770
Escherichia coli
?
3.6.1.41
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
246770
Escherichia coli EM20031
ADP
-
246770
Escherichia coli EM20031
?
3.6.1.41
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
-
246770
Escherichia coli
GTP
-
-
-
?
3.6.1.41
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
-
246770
Escherichia coli EM20031
GTP
-
-
-
?
3.6.1.41
P1,P6-di(adenosine-5')hexaphosphate + H2O
-
246770
Escherichia coli
ADP + adenosine 5'-tetraphosphate
-
246770
Escherichia coli
?
3.6.1.41
P1,P6-di(adenosine-5')hexaphosphate + H2O
-
246770
Escherichia coli EM20031
ADP + adenosine 5'-tetraphosphate
-
246770
Escherichia coli EM20031
?
Subunits
EC Number
Subunits
Commentary
Organism
3.6.1.41
monomer
1 * 34000, SDS-PAGE
Escherichia coli
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6.1.41
250
-
P1,P4-bis(5'-adenosyl) tetraphosphate
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.41
Zn2+
exposure prior to the assay
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.41
0.012
-
P1,P4-bis(5'-adenosyl) tetraphosphate
with Mn2+
Escherichia coli
3.6.1.41
0.022
-
P1,P4-bis(5'-adenosyl) tetraphosphate
with Co2+
Escherichia coli
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.41
Ca2+
no effect
Escherichia coli
3.6.1.41
Co2+
2500fold stimulation
Escherichia coli
3.6.1.41
Mn2+
900fold stimulation
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.41
33000
-
gel filtration
Escherichia coli
3.6.1.41
34000
-
1 * 34000, SDS-PAGE
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.41
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.6.1.41
additional information
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.41
adenosine 5'-P(1)-tetraphosphate-P(4)-5'''-cytidine + H2O
-
246770
Escherichia coli
ADP + CDP
-
246770
Escherichia coli
?
3.6.1.41
adenosine 5'-P(1)-triphosphate-P(3)-5''-cytidine + H2O
-
246770
Escherichia coli
ADP + CMP + CDP + AMP
-
246770
Escherichia coli
?
3.6.1.41
adenosine 5'-P(1)-triphosphate-P(3)-5''-guanosine + H2O
-
246770
Escherichia coli
ADP + GMP
-
246770
Escherichia coli
?
3.6.1.41
Ap3A + H2O
-
246770
Escherichia coli
ADP + AMP
-
-
-
?
3.6.1.41
Ap3A + H2O
-
246770
Escherichia coli EM20031
ADP + AMP
-
-
-
?
3.6.1.41
Ap4 + H2O
-
246770
Escherichia coli
?
-
-
-
?
3.6.1.41
Ap4G + H2O
-
246770
Escherichia coli
ADP + GDP
-
246770
Escherichia coli
?
3.6.1.41
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
-
246770
Escherichia coli
ADP + ATP
-
246770
Escherichia coli
?
3.6.1.41
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
-
246770
Escherichia coli EM20031
ADP + ATP
-
246770
Escherichia coli EM20031
?
3.6.1.41
Gp3C + H2O
-
246770
Escherichia coli
CDP + GMP
-
246770
Escherichia coli
?
3.6.1.41
Gp3G + H2O
-
246770
Escherichia coli
guanosine 3'-diphosphate + guanosine 5'-phosphate
-
-
-
?
3.6.1.41
P1,P4-bis(5'-(2'-deoxy)adenosyl)tetraphosphate + H2O
-
246770
Escherichia coli
2 dADP
-
246770
Escherichia coli
?
3.6.1.41
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
246770
Escherichia coli
ADP
-
246770
Escherichia coli
?
3.6.1.41
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
246770
Escherichia coli EM20031
ADP
-
246770
Escherichia coli EM20031
?
3.6.1.41
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
-
246770
Escherichia coli
GTP
-
-
-
?
3.6.1.41
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
-
246770
Escherichia coli EM20031
GTP
-
-
-
?
3.6.1.41
P1,P6-di(adenosine-5')hexaphosphate + H2O
-
246770
Escherichia coli
ADP + adenosine 5'-tetraphosphate
-
246770
Escherichia coli
?
3.6.1.41
P1,P6-di(adenosine-5')hexaphosphate + H2O
-
246770
Escherichia coli EM20031
ADP + adenosine 5'-tetraphosphate
-
246770
Escherichia coli EM20031
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.6.1.41
monomer
1 * 34000, SDS-PAGE
Escherichia coli
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6.1.41
250
-
P1,P4-bis(5'-adenosyl) tetraphosphate
-
Escherichia coli