BRENDA - Enzyme Database

Catabolism of diadenosine 5,5-P1,P4-tetraphosphate in procaryotes. Purification and properties of diadenosine 5,5-P1,P4-tetraphosphate (symmetrical) pyrophosphohydrolase from Escherichia coli K12

Guranowski, A.; Jakubowski, H.; Holler, E.; J. Biol. Chem. 258, 14784-14789 (1983)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.41
1,10-phenanthroline
-
Escherichia coli
3.6.1.41
Cu2+
-
Acidaminococcus fermentans
3.6.1.41
Cu2+
no effect below 0.03 mM, inhibition above
Escherichia coli
3.6.1.41
Cu2+
-
Pyrodictium occultum
3.6.1.41
Fe2+
no effect below 0.03 mM, inhibition above
Acidaminococcus fermentans
3.6.1.41
Fe2+
no effect below 0.03 mM, inhibition above
Escherichia coli
3.6.1.41
Fe2+
no effect below 0.03 mM, inhibition above
Pyrodictium occultum
3.6.1.41
Zn2+
no effect below 0.03 mM, inhibition above
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.41
0.025
-
P1,P4-bis(5'-adenosyl) tetraphosphate
-
Escherichia coli
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.41
Ca2+
weak stimulation in absence of other divalent metal ions, high synergistic stimulation in combination with Co2+ at its suboptimal concentration
Escherichia coli
3.6.1.41
Cd2+
0.002-0.005 mM, 3.5fold stimulation
Escherichia coli
3.6.1.41
Co2+
0.1-0.5 mM, 100fold increase of activity
Escherichia coli
3.6.1.41
Mg2+
weak stimulation in absence of other divalent metal ions, high synergistic stimulation in combination with Co2+ at its suboptimal concentrations
Escherichia coli
3.6.1.41
Mn2+
0.2-0.5 mM, 8fold stimulation
Escherichia coli
3.6.1.41
Ni2+
0.004-0.008 mM, 3.5fold stimulation
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.41
27000
-
gel filtration
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.41
Acidaminococcus fermentans
-
-
-
3.6.1.41
Escherichia coli
-
K12
-
3.6.1.41
Pyrodictium occultum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.6.1.41
-
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.6.1.41
0.062
-
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.41
Ap3A + H2O
-
246765
Escherichia coli
ADP + AMP
-
-
-
?
3.6.1.41
Ap4 + H2O
-
246765
Escherichia coli
?
-
-
-
?
3.6.1.41
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
-
246765
Escherichia coli
ADP + ATP
-
-
-
?
3.6.1.41
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
246765
Escherichia coli
ADP
-
246765
Escherichia coli
?
3.6.1.41
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
246765
Acidaminococcus fermentans
ADP
-
246765
Acidaminococcus fermentans
?
3.6.1.41
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
246765
Pyrodictium occultum
ADP
-
246765
Pyrodictium occultum
?
3.6.1.41
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
-
246765
Escherichia coli
GTP
-
-
-
?
3.6.1.41
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
-
246765
Acidaminococcus fermentans
GTP
-
-
-
?
3.6.1.41
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
-
246765
Pyrodictium occultum
GTP
-
-
-
?
3.6.1.41
P1,P6-di(adenosine-5')hexaphosphate + H2O
-
246765
Escherichia coli
ADP + adenosine 5'-tetraphosphate
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.41
8.3
-
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.41
1,10-phenanthroline
-
Escherichia coli
3.6.1.41
Cu2+
-
Acidaminococcus fermentans
3.6.1.41
Cu2+
no effect below 0.03 mM, inhibition above
Escherichia coli
3.6.1.41
Cu2+
-
Pyrodictium occultum
3.6.1.41
Fe2+
no effect below 0.03 mM, inhibition above
Acidaminococcus fermentans
3.6.1.41
Fe2+
no effect below 0.03 mM, inhibition above
Escherichia coli
3.6.1.41
Fe2+
no effect below 0.03 mM, inhibition above
Pyrodictium occultum
3.6.1.41
Zn2+
no effect below 0.03 mM, inhibition above
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.41
0.025
-
P1,P4-bis(5'-adenosyl) tetraphosphate
-
Escherichia coli
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.41
Ca2+
weak stimulation in absence of other divalent metal ions, high synergistic stimulation in combination with Co2+ at its suboptimal concentration
Escherichia coli
3.6.1.41
Cd2+
0.002-0.005 mM, 3.5fold stimulation
Escherichia coli
3.6.1.41
Co2+
0.1-0.5 mM, 100fold increase of activity
Escherichia coli
3.6.1.41
Mg2+
weak stimulation in absence of other divalent metal ions, high synergistic stimulation in combination with Co2+ at its suboptimal concentrations
Escherichia coli
3.6.1.41
Mn2+
0.2-0.5 mM, 8fold stimulation
Escherichia coli
3.6.1.41
Ni2+
0.004-0.008 mM, 3.5fold stimulation
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.41
27000
-
gel filtration
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.41
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.6.1.41
0.062
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.41
Ap3A + H2O
-
246765
Escherichia coli
ADP + AMP
-
-
-
?
3.6.1.41
Ap4 + H2O
-
246765
Escherichia coli
?
-
-
-
?
3.6.1.41
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
-
246765
Escherichia coli
ADP + ATP
-
-
-
?
3.6.1.41
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
246765
Escherichia coli
ADP
-
246765
Escherichia coli
?
3.6.1.41
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
246765
Acidaminococcus fermentans
ADP
-
246765
Acidaminococcus fermentans
?
3.6.1.41
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
246765
Pyrodictium occultum
ADP
-
246765
Pyrodictium occultum
?
3.6.1.41
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
-
246765
Escherichia coli
GTP
-
-
-
?
3.6.1.41
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
-
246765
Acidaminococcus fermentans
GTP
-
-
-
?
3.6.1.41
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
-
246765
Pyrodictium occultum
GTP
-
-
-
?
3.6.1.41
P1,P6-di(adenosine-5')hexaphosphate + H2O
-
246765
Escherichia coli
ADP + adenosine 5'-tetraphosphate
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.41
8.3
-
-
Escherichia coli