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Literature summary extracted from

  • Guranowski, A.; Jakubowski, H.; Holler, E.
    Catabolism of diadenosine 5,5-P1,P4-tetraphosphate in procaryotes. Purification and properties of diadenosine 5,5-P1,P4-tetraphosphate (symmetrical) pyrophosphohydrolase from Escherichia coli K12 (1983), J. Biol. Chem., 258, 14784-14789.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.41 1,10-phenanthroline
-
 Escherichia coli
3.6.1.41 Cu2+
-
 Acidaminococcus fermentans
3.6.1.41 Cu2+ no effect below 0.03 mM, inhibition above Escherichia coli
3.6.1.41 Cu2+
-
 Pyrodictium occultum
3.6.1.41 Fe2+ no effect below 0.03 mM, inhibition above Acidaminococcus fermentans
3.6.1.41 Fe2+ no effect below 0.03 mM, inhibition above Escherichia coli
3.6.1.41 Fe2+ no effect below 0.03 mM, inhibition above Pyrodictium occultum
3.6.1.41 Zn2+ no effect below 0.03 mM, inhibition above Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.41 0.025
-
 P1,P4-bis(5'-adenosyl) tetraphosphate
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.41 Ca2+ weak stimulation in absence of other divalent metal ions, high synergistic stimulation in combination with Co2+ at its suboptimal concentration Escherichia coli
3.6.1.41 Cd2+ 0.002-0.005 mM, 3.5fold stimulation Escherichia coli
3.6.1.41 Co2+ 0.1-0.5 mM, 100fold increase of activity Escherichia coli
3.6.1.41 Mg2+ weak stimulation in absence of other divalent metal ions, high synergistic stimulation in combination with Co2+ at its suboptimal concentrations Escherichia coli
3.6.1.41 Mn2+ 0.2-0.5 mM, 8fold stimulation Escherichia coli
3.6.1.41 Ni2+ 0.004-0.008 mM, 3.5fold stimulation Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.41 27000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.41 Acidaminococcus fermentans
-
-
-
3.6.1.41 Escherichia coli
-
 K12
-
3.6.1.41 Pyrodictium occultum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.41
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.41 0.062
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.41 Ap3A + H2O
-
 Escherichia coli ADP + AMP
-
 ?
3.6.1.41 Ap4 + H2O
-
 Escherichia coli ?
-
 ?
3.6.1.41 diadenosine 5',5''-P1,P5-pentaphosphate + H2O
-
 Escherichia coli ADP + ATP
-
 ?
3.6.1.41 P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
 Escherichia coli ADP
-
 ?
3.6.1.41 P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
 Acidaminococcus fermentans ADP
-
 ?
3.6.1.41 P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
 Pyrodictium occultum ADP
-
 ?
3.6.1.41 P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
-
 Escherichia coli GTP
-
 ?
3.6.1.41 P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
-
 Acidaminococcus fermentans GTP
-
 ?
3.6.1.41 P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
-
 Pyrodictium occultum GTP
-
 ?
3.6.1.41 P1,P6-di(adenosine-5')hexaphosphate + H2O
-
 Escherichia coli ADP + adenosine 5'-tetraphosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.41 8.3
-
-
 Escherichia coli