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Literature summary extracted from

  • Wittliff, J.L.; Airth, R.L.
    Thiaminase II (thiamine hydrolase, EC 3.5.99.2) (1971), Methods Enzymol., 18, 234-238.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.99.2 EDTA in a concentration range of 0.02 mM to 1000 mM Aneurinibacillus aneurinilyticus
3.5.99.2 L-cysteine in a concentration range of 0.02 mM to 1000 mM Aneurinibacillus aneurinilyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.99.2 Cu2+ 0.02 mM Aneurinibacillus aneurinilyticus
3.5.99.2 Fe2+ 0.02 mM Aneurinibacillus aneurinilyticus
3.5.99.2 additional information inhibited by many aromatic and heterocyclic amines Aneurinibacillus aneurinilyticus
3.5.99.2 p-chloromercuribenzoate 0.02 mM Aneurinibacillus aneurinilyticus
3.5.99.2 Zn2+ 0.02 mM Aneurinibacillus aneurinilyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.99.2 0.003
-
 thiamine
-
 Aneurinibacillus aneurinilyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.99.2 100000
-
 ultracentrifugation Aneurinibacillus aneurinilyticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.99.2 thiamine + H2O Candida sp. (in: Saccharomycetales)
-
 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?
3.5.99.2 thiamine + H2O Geotrichum candidum
-
 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?
3.5.99.2 thiamine + H2O Aneurinibacillus aneurinilyticus
-
 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?
3.5.99.2 thiamine + H2O Trichosporon aneurinolyticum
-
 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.99.2 Aneurinibacillus aneurinilyticus
-
-
-
3.5.99.2 Candida sp. (in: Saccharomycetales)
-
-
-
3.5.99.2 Geotrichum candidum
-
-
-
3.5.99.2 Trichosporon aneurinolyticum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.99.2
-
 Aneurinibacillus aneurinilyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.99.2 thiamine + H2O
-
 Candida sp. (in: Saccharomycetales) 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?
3.5.99.2 thiamine + H2O
-
 Geotrichum candidum 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?
3.5.99.2 thiamine + H2O
-
 Aneurinibacillus aneurinilyticus 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?
3.5.99.2 thiamine + H2O
-
 Trichosporon aneurinolyticum 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.99.2 monomer 1 * 100000, SDS-PAGE Aneurinibacillus aneurinilyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.99.2 30 40
-
 Candida sp. (in: Saccharomycetales)
3.5.99.2 40 45
-
 Trichosporon aneurinolyticum
3.5.99.2 60
-
-
 Geotrichum candidum
3.5.99.2 60
-
-
 Aneurinibacillus aneurinilyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.99.2 60
-
 enzyme inactivated by heating for 10 min Candida sp. (in: Saccharomycetales)
3.5.99.2 60
-
 enzyme inactivated by heating for 10 min Trichosporon aneurinolyticum
3.5.99.2 80
-
 enzyme inactivated by heating for 10 min Geotrichum candidum
3.5.99.2 80
-
 enzyme inactivated by heating for 10 min Aneurinibacillus aneurinilyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.99.2 7
-
-
 Candida sp. (in: Saccharomycetales)
3.5.99.2 7
-
-
 Geotrichum candidum
3.5.99.2 7
-
-
 Trichosporon aneurinolyticum
3.5.99.2 8.6
-
-
 Aneurinibacillus aneurinilyticus