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Literature summary extracted from

  • Breitung, J.; Schmitz, R.A.; Stetter, K.O.; Thauer, R.K.
    N5,N10-Methenyltetrahydromethanopterin cyclohydrolase from the extreme thermophile Methanopyrus kandleri: increase of the catalytic efficiency (kcat/KM) and thermostability in the presence of salts (1991), Arch. Microbiol., 156, 517-524.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.5.4.27 addition of K2HPO4 or other salts prevents inactivation, relatively low concentrations of the salt, below 0.1 mM, are required for the stabilization, potassium salts being more effective than ammonium and sodium salts, at concentrations above 0.5 M, which are required for maximal activity the thermostability decreases again Methanopyrus kandleri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.27 additional information
-
 additional information effect of salts on Km Methanopyrus kandleri
3.5.4.27 0.1
-
 N5,N10-methenyl-5,6,7,8-tetrahydromethanopterin 1 M K2HPO4 Methanopyrus kandleri
3.5.4.27 0.14
-
 N5,N10-methenyl-5,6,7,8-tetrahydromethanopterin 0.6 M K2HPO4 Methanopyrus kandleri
3.5.4.27 0.2
-
 N5,N10-methenyl-5,6,7,8-tetrahydromethanopterin 0.3 M K2HPO4 Methanopyrus kandleri
3.5.4.27 2
-
 N5,N10-methenyl-5,6,7,8-tetrahydromethanopterin
-
 Methanopyrus kandleri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.27 (NH4)2SO4 1.5 M, stimulates Methanopyrus kandleri
3.5.4.27 ammonium sulfate 1.5 M, maximal 100fold stimulation Methanopyrus kandleri
3.5.4.27 K2HPO4 maximal activation by 1.5 M K2HPO4, 1 M Na2HPO4, or 1 M Na2SO4 Methanopyrus kandleri
3.5.4.27 K2HPO4 200fold increase of activity at 1.5 M Methanopyrus kandleri
3.5.4.27 K2SO4 0.8 M stimulates Methanopyrus kandleri
3.5.4.27 K2SO4 0.8 M, maximal 100fold stimulation Methanopyrus kandleri
3.5.4.27 KCl 2.5 M stimulates Methanopyrus kandleri
3.5.4.27 KCl 2.5 M, maximal 100fold stimulation Methanopyrus kandleri
3.5.4.27 Na2HPO4 maximal activation by 1.5 M K2HPO4, 1 M Na2HPO4 or 1 M Na2SO4 Methanopyrus kandleri
3.5.4.27 Na2SO4 maximal activation by 1.5 M K2HPO4, 1 M Na2HPO4 or 1 M Na2SO4 Methanopyrus kandleri
3.5.4.27 NaCl 2.0 M, stimulates Methanopyrus kandleri
3.5.4.27 NaCl 2 M, maximal 100fold stimulation Methanopyrus kandleri
3.5.4.27 NH4Cl optimal concentration is 0.3 M Methanopyrus kandleri
3.5.4.27 Sodium phosphate 1 M, 200fold stimulation Methanopyrus kandleri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.27 41500
-
 1 * 41500, SDS-PAGE Methanopyrus kandleri
3.5.4.27 42000
-
 non-denaturing PAGE Methanopyrus kandleri

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.27 Methanopyrus kandleri
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.5.4.27 the purified enzyme is more resistant to inactivation by O2 than the enzyme in cell extract Methanopyrus kandleri

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.27
-
 Methanopyrus kandleri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.27 5026
-
-
 Methanopyrus kandleri

Storage Stability

EC Number Storage Stability Organism
3.5.4.27 -20°C, under N2, 50 mM Tris/HCl buffer, pH 7, containing 1.3 M KCl, no loss of activity for several months Methanopyrus kandleri
3.5.4.27 -20°C, under N2, in 50 mM Tris/HCl, pH 7, containing 1.3 M KCl, stable for several months Methanopyrus kandleri
3.5.4.27 0°C, both under anaeobic and aerobic conditions, the enzyme also loses very little activity within 24 h Methanopyrus kandleri
3.5.4.27 0°C, both under anaerobic and anaerobic conditions, stable for 24 h Methanopyrus kandleri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
-
 Methanopyrus kandleri N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 r

Subunits

EC Number Subunits Comment Organism
3.5.4.27 monomer 1 * 41500, SDS-PAGE Methanopyrus kandleri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.27 95
-
-
 Methanopyrus kandleri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.27 90
-
 thermostable up to 90°C at 0.04 M K2HPO4 Methanopyrus kandleri
3.5.4.27 90
-
 60 min, 50 mM tricine/KOH, pH 8, stable Methanopyrus kandleri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.27 8
-
-
 Methanopyrus kandleri