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Literature summary extracted from

  • Adams, E.
    Enzymes and intermediates of hydroxyproline degradation (1971), Methods Enzymol., 17, 266-306.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.22 62000
-
 ultracentrifugation Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.22 L-1-pyrroline-4-hydroxy-2-carboxylate + H2O Pseudomonas putida
-
 2,5-dioxopentanoate + NH3
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.22 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.22
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.22 4.78
-
-
 Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
3.5.4.22 -15°C, fraction II, at least one month Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.22 D-1-pyrroline-4-hydroxy-2-carboxylate + H2O
-
 Pseudomonas putida 2,5-dioxopentanoate + NH3
-
 ir
3.5.4.22 L-1-pyrroline-4-hydroxy-2-carboxylate + H2O
-
 Pseudomonas putida 2,5-dioxopentanoate + NH3
-
 ir

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.22 70
-
 fraction III, 5 min, slight loss in activity Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.22 7
-
-
 Pseudomonas putida