BRENDA - Enzyme Database

Purification and properties of agmatine ureohydrolase, a putrescine biosynthetic enzyme in Escherichia coli

Satishchandran, C.; Boyle S.M.; J. Bacteriol. 165, 843-848 (1986)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.11
arginine
competitive inhibitor
Escherichia coli
3.5.3.11
EDTA
1 mM causes 53% inhibition
Escherichia coli
3.5.3.11
EGTA
1 mM causes 74% inhibition
Escherichia coli
3.5.3.11
ornithine
noncompetitive inhibitor
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.11
1.2
-
agmatine
-
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.11
33409
-
2 * 38000, SDS-PAGE, 2 * 33409, deduced from sequence
Escherichia coli
3.5.3.11
38000
-
2 * 38000, SDS-PAGE, 2 * 33409, deduced from sequence
Escherichia coli
3.5.3.11
80000
-
gel filtration
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.3.11
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.3.11
-
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
3.5.3.11
-80°C, at least three months
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.11
agmatine + H2O
-
246681
Escherichia coli
putrescine + urea
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.5.3.11
dimer
2 * 38000, SDS-PAGE, 2 * 33409, deduced from sequence
Escherichia coli
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.3.11
42
-
-
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.11
7.3
-
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.11
arginine
competitive inhibitor
Escherichia coli
3.5.3.11
EDTA
1 mM causes 53% inhibition
Escherichia coli
3.5.3.11
EGTA
1 mM causes 74% inhibition
Escherichia coli
3.5.3.11
ornithine
noncompetitive inhibitor
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.11
1.2
-
agmatine
-
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.11
33409
-
2 * 38000, SDS-PAGE, 2 * 33409, deduced from sequence
Escherichia coli
3.5.3.11
38000
-
2 * 38000, SDS-PAGE, 2 * 33409, deduced from sequence
Escherichia coli
3.5.3.11
80000
-
gel filtration
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.11
-
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.3.11
-80°C, at least three months
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.11
agmatine + H2O
-
246681
Escherichia coli
putrescine + urea
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.3.11
dimer
2 * 38000, SDS-PAGE, 2 * 33409, deduced from sequence
Escherichia coli
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.3.11
42
-
-
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.11
7.3
-
-
Escherichia coli