BRENDA - Enzyme Database

Purification and properties of agmatine amidinohydrolase of Evernia prunastri

Vicente, C.; Legaz, M.E.; Physiol. Plant. 55, 335-339 (1982)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.3.11
L-arginine
but it inhibits at concentrations about 14 mM agmatine
Evernia prunastri
3.5.3.11
L-ornithine
but it inhibits at concentrations about 14 mM agmatine
Evernia prunastri
3.5.3.11
putrescine
but it inhibits at concentrations about 14 mM agmatine
Evernia prunastri
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.11
Chloroatranorin
12.6 mM
Evernia prunastri
3.5.3.11
everinic acid
18.5 mM
Evernia prunastri
3.5.3.11
Urea
-
Evernia prunastri
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.11
6.4
-
agmatine
-
Evernia prunastri
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.11
320000
-
gel filtration
Evernia prunastri
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.11
agmatine + H2O
Evernia prunastri
-
putrescine + urea
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.3.11
Evernia prunastri
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.3.11
-
Evernia prunastri
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.3.11
thallus
-
Evernia prunastri
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.11
agmatine + H2O
-
246677
Evernia prunastri
putrescine + urea
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.3.11
35
40
-
Evernia prunastri
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.3.11
-
-
6% of activity retained
Evernia prunastri
3.5.3.11
70
-
20% of activity retained
Evernia prunastri
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.11
6.9
-
-
Evernia prunastri
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.3.11
L-arginine
but it inhibits at concentrations about 14 mM agmatine
Evernia prunastri
3.5.3.11
L-ornithine
but it inhibits at concentrations about 14 mM agmatine
Evernia prunastri
3.5.3.11
putrescine
but it inhibits at concentrations about 14 mM agmatine
Evernia prunastri
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.11
Chloroatranorin
12.6 mM
Evernia prunastri
3.5.3.11
everinic acid
18.5 mM
Evernia prunastri
3.5.3.11
Urea
-
Evernia prunastri
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.11
6.4
-
agmatine
-
Evernia prunastri
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.11
320000
-
gel filtration
Evernia prunastri
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.11
agmatine + H2O
Evernia prunastri
-
putrescine + urea
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.11
-
Evernia prunastri
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.3.11
thallus
-
Evernia prunastri
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.11
agmatine + H2O
-
246677
Evernia prunastri
putrescine + urea
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.3.11
35
40
-
Evernia prunastri
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.3.11
-
-
6% of activity retained
Evernia prunastri
3.5.3.11
70
-
20% of activity retained
Evernia prunastri
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.11
6.9
-
-
Evernia prunastri