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Literature summary extracted from

  • Kennedy, J.
    Dihydroorotase from rat liver: purification, properties and regulatory role in pyrimidine biosynthesis (1974), Arch. Biochem. Biophys., 160, 358-365.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.3 Ag2+ 0.2 mM, 63% inhibition Rattus norvegicus
3.5.2.3 Cd2+ 0.2 mM, 12% inhibition Rattus norvegicus
3.5.2.3 Cu2+ 0.2 mM, 33% inhibition Rattus norvegicus
3.5.2.3 Hg2+ 0.2 mM, 87% inhibition Rattus norvegicus
3.5.2.3 Zn2+ 0.2 mM, 18% inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.3 0.04
-
 L-dihydroorotate
-
 Rattus norvegicus
3.5.2.3 0.12
-
 L-carbamoylaspartate
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.2.3 cytosol
-
 Rattus norvegicus 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.3 N-carbamoyl-L-aspartate Rattus norvegicus
-
 L-dihydroorotate + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.3 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.3
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.2.3 liver
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
3.5.2.3 -20°C, 1 month, 40% loss in activity Rattus norvegicus
3.5.2.3 4°C, 2 months, partially purified preparation Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.3 L-carbamoylaspartate
-
 Rattus norvegicus L-dihydroorotate + H2O
-
 r
3.5.2.3 N-carbamoyl-L-aspartate
-
 Rattus norvegicus L-dihydroorotate + H2O
-
 r