Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Taylor, W.H.; Taylor, M.L.; Balch, W.E.; Gilchrist, P.S.
    Purification and properties of dihydroorotase, a zinc-containing metalloenzyme in Clostridium oroticum (1976), J. Bacteriol., 127, 863-873.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.3 phosphate 300 mM caused 84% inhibition Faecalicatena orotica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.3 0.07
-
 L-dihydroorotate
-
 Faecalicatena orotica
3.5.2.3 0.13
-
 L-carbamoylaspartate
-
 Faecalicatena orotica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.3 Zn2+ zinc metalloprotein, contains 4 g Zn/mol of dimer Faecalicatena orotica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.3 55000
-
 2 * 55000, SDS-PAGE Faecalicatena orotica
3.5.2.3 110000
-
 gel filtration Faecalicatena orotica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.3 N-carbamoyl-L-aspartate Faecalicatena orotica
-
 L-dihydroorotate + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.3 Faecalicatena orotica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.3
-
 Faecalicatena orotica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.3 0.032
-
-
 Faecalicatena orotica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.3 L-carbamoylaspartate
-
 Faecalicatena orotica L-dihydroorotate + H2O
-
 r
3.5.2.3 N-carbamoyl-L-aspartate
-
 Faecalicatena orotica L-dihydroorotate + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
3.5.2.3 dimer 2 * 55000, SDS-PAGE Faecalicatena orotica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.3 6
-
-
 Faecalicatena orotica
3.5.2.3 8
-
-
 Faecalicatena orotica