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Literature summary extracted from

  • Karns, J.S.
    Gene sequence and properties of an s-triazine ring-cleavage enzyme from Pseudomonas sp. strain NRRLB-12227 (1999), Appl. Environ. Microbiol., 65, 3512-3517.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.2.15 agriculture cleavage of the s-triazine ring is an important step in the mineralization of s-triazine compounds, extensive used as herbicides or pesticides in agriculture worldwide, and hence in their complete removal from environment Pseudomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.15 strain A, expression and overproduction in Escherichia coli DH5-alpha, BRL Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.15 Barbituric acid 2,4,6-trihydroxypyrimidine, strong competitive inhibitor, Ki less than 0.0001 mM Pseudomonas sp.
3.5.2.15 Co2+ strain A, 1 mM: slight inhibition Pseudomonas sp.
3.5.2.15 Cu2+ strain A, 1 mM: slight inhibition Pseudomonas sp.
3.5.2.15 Fe2+ strain A, 1 mM: slight inhibition Pseudomonas sp.
3.5.2.15 additional information strain A, no inhibition by 0.05 mM of ammeline, 2,4-diamino-6-hydroxy-s-triazine, ammelide, 2-amino-4,6-dihydroxy-s-triazine, uracil, 2,4-dihydroxypyrimidine, 5,6-dihydrouracil, cytosine, 4-amino-2-hydroxypyrimidine, 2,4,5-trihydroxypyrimidine; strain A, no inhibition by 1 mM EDTA, 1 mM Mg2+ or 1 mM Mn2+ as either sulfate or chloride salts Pseudomonas sp.
3.5.2.15 Zn2+ strain A, 1 mM: 100fold inhibition Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.15 additional information
-
 additional information strain A, kinetic analyses and kinetic data Pseudomonas sp.
3.5.2.15 0.05
-
 cyanuric acid strain A, pH 8.0 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.15 additional information strain A, divalent cations not required, although the possibility that a tightly bound metal ion is present in enzyme cannot be ruled out, no metal analysis of enzyme is performed directly Pseudomonas sp.
3.5.2.15 additional information strain A, no effect, no activation by 1 mM Mg2+ or 1 mM Mn2+ as either sulfate or chloride salts Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.15 39400
-
 strain A, 4 * 39400, calculated from DNA sequence Pseudomonas sp.
3.5.2.15 40000
-
 strain A, 4 * 40000, SDS-PAGE Pseudomonas sp.
3.5.2.15 185000
-
 strain A, SW300 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.15 cyanuric acid + H2O Pseudomonas sp. 2,4,6-trihydroxy-1,3,5-triazine biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O Pseudomonas sp. 1,3,5-triazine-2,4,6(1H,3H,5H)-trione biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O Pseudomonas sp. 2,4,6-trihydroxy-s-triazine biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O Pseudomonas sp. first enzyme of degradative pathway of cyanuric acid biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O Pseudomonas sp. involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O Pseudomonas sp. herterocyclic ring cleavage biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O Pseudomonas sp. involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine biuret + CO2
-
 ?
3.5.2.15 additional information Pseudomonas sp. involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria ?
-
 ?
3.5.2.15 additional information Pseudomonas sp. involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine ?
-
 ?
3.5.2.15 additional information Pseudomonas sp. A involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria ?
-
 ?
3.5.2.15 additional information Pseudomonas sp. A involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.15 Pseudomonas sp.
-
 NRRLB-12227
-
3.5.2.15 Pseudomonas sp. A
-
 NRRLB-12227
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.15 strain A, NRRLB-12227, from recombinant Escherichia coli DH5-alpha containing the cloned trzD gene Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.15 109.5
-
 strain A, from recombinant Escherichia coli containing trzD gene Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.15 cyanuric acid + H2O high substrate specificity Pseudomonas sp. biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O 2,4,6-trihydroxy-1,3,5-triazine Pseudomonas sp. biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O 1,3,5-triazine-2,4,6(1H,3H,5H)-trione Pseudomonas sp. biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O 2,4,6-trihydroxy-s-triazine Pseudomonas sp. biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O heterocyclic ring cleavage Pseudomonas sp. biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O first enzyme of degradative pathway of cyanuric acid Pseudomonas sp. biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria Pseudomonas sp. biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O herterocyclic ring cleavage Pseudomonas sp. biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine Pseudomonas sp. biuret + CO2
-
 ?
3.5.2.15 cyanuric acid + H2O high substrate specificity Pseudomonas sp. A biuret + CO2
-
 ?
3.5.2.15 additional information strain A, no activity with ammeline, 2,4-diamino-6-hydroxy-s-triazine, ammelide, 2-amino-4,6-dihydroxy-s-triazine, uracil, 2,4-dihydroxypyrimidine, 5,6-dihydrouracil, cytosine, 4-amino-2-hydroxypyrimidine, 2,4,5-trihydroxypyrimidine and barbituric acid, 2,4,6-trihydroxypyrimidine Pseudomonas sp. ?
-
 ?
3.5.2.15 additional information strain A, high substrate specificity, no activity with any other s-triazine or hydroxypyrimidine compound Pseudomonas sp. ?
-
 ?
3.5.2.15 additional information involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria Pseudomonas sp. ?
-
 ?
3.5.2.15 additional information involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine Pseudomonas sp. ?
-
 ?
3.5.2.15 additional information strain A, no activity with ammeline, 2,4-diamino-6-hydroxy-s-triazine, ammelide, 2-amino-4,6-dihydroxy-s-triazine, uracil, 2,4-dihydroxypyrimidine, 5,6-dihydrouracil, cytosine, 4-amino-2-hydroxypyrimidine, 2,4,5-trihydroxypyrimidine and barbituric acid, 2,4,6-trihydroxypyrimidine Pseudomonas sp. A ?
-
 ?
3.5.2.15 additional information strain A, high substrate specificity, no activity with any other s-triazine or hydroxypyrimidine compound Pseudomonas sp. A ?
-
 ?
3.5.2.15 additional information involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria Pseudomonas sp. A ?
-
 ?
3.5.2.15 additional information involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine Pseudomonas sp. A ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.15 tetramer strain A, 4 * 40000, SDS-PAGE Pseudomonas sp.
3.5.2.15 tetramer strain A, 4 * 39400, calculated from DNA sequence Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.15 45 50 strain A Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.15 250
-
 cyanuric acid strain A, pH 8.0 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.15 8 8.5 strain A Pseudomonas sp.