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Literature summary extracted from
- Nucleoside triphosphatase activity of an ATP-dependent enzyme, N-methylhydantoin amidohydrolase (1995), Biosci. Biotechnol. Biochem., 59, 1737-1739.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.2.14 | N-methylhydantoin | inhibits the hydrolysis of other nucleoside triphosphates than ATP and dATP | Pseudomonas putida |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.2.14 | K+ | - |
Pseudomonas putida | |
| 3.5.2.14 | Mg2+ | - |
Pseudomonas putida | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.2.14 | Pseudomonas putida | - |
77 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.2.14 | ATP + dihydrouracil + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 3.5.2.14 | CTP + dihydrouracil + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 3.5.2.14 | dATP + dihydrouracil + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 3.5.2.14 | dATP + N-methylimidazolidine-2,4-dione + H2O | - |
Pseudomonas putida | dADP + phosphate + N-carbamoylsarcosine | - |
? | |
| 3.5.2.14 | dGTP + dihydrouracil + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 3.5.2.14 | dITP + dihydrouracil + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 3.5.2.14 | dUTP + dihydrouracil + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 3.5.2.14 | GTP + dihydrouracil + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 3.5.2.14 | ITP + dihydrouracil + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 3.5.2.14 | UTP + dihydrouracil + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 3.5.2.14 | XTP + dihydrouracil + H2O | - |
Pseudomonas putida | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.14 | 45 | - |
ATP in presence of N-methylhydantoin | Pseudomonas putida |
| 3.5.2.14 | 50 | - |
CTP in absence of N-methylhydantoin | Pseudomonas putida |
| 3.5.2.14 | 55 | - |
CTP in presence of N-methylhydantoin | Pseudomonas putida |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.14 | 37 | 70 | CTP-hydrolysis | Pseudomonas putida |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.14 | 60 | - |
about 70% of initial activity remains | Pseudomonas putida |
| 3.5.2.14 | 70 | - |
about 20% of initial activity remains | Pseudomonas putida |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.14 | 7.4 | 9 | - |
Pseudomonas putida |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.14 | 5 | - |
loss of 25% in activity | Pseudomonas putida |
| 3.5.2.14 | 10.6 | - |
loss of 30% in activity | Pseudomonas putida |
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