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Purification and properties of an aromatic amidase from Pseudomonas sp. GDI 211

Kgayama, T.; Ohe, T.; Agric. Biol. Chem. 54, 2565-2571 (1990)
No PubMed abstract available

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.5.1.19
enzyme is stable in buffer solution containing Co2+ and Ni2+
Pseudomonas sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.19
Cu2+
-
Pseudomonas sp.
3.5.1.19
Hg2+
-
Pseudomonas sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.005
-
nicotinamide
-
Pseudomonas sp.
3.5.1.19
0.056
-
Pyrazinamide
-
Pseudomonas sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.19
Co2+
enhances activity
Pseudomonas sp.
3.5.1.19
Mn2+
enhances activity
Pseudomonas sp.
3.5.1.19
Ni2+
enhances activity
Pseudomonas sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.19
26000
-
2 * 26000, SDS-PAGE
Pseudomonas sp.
3.5.1.19
43000
-
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.19
nicotinamide + H2O
Pseudomonas sp.
-
nicotinate + NH3
-
-
?
3.5.1.19
nicotinamide + H2O
Pseudomonas sp. GDI 211
-
nicotinate + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.19
Pseudomonas sp.
-
-
-
3.5.1.19
Pseudomonas sp. GDI 211
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.19
-
Pseudomonas sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.19
37.6
-
-
Pseudomonas sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
246588
Pseudomonas sp.
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
59.5% of the activity with pyrazinamide
246588
Pseudomonas sp.
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
-
246588
Pseudomonas sp. GDI 211
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
59.5% of the activity with pyrazinamide
246588
Pseudomonas sp. GDI 211
nicotinate + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
246588
Pseudomonas sp.
pyrazinoic acid + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
246588
Pseudomonas sp. GDI 211
pyrazinoic acid + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.19
dimer
2 * 26000, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.19
75
-
-
Pseudomonas sp.
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.1.19
55
80
about 35% of maximal activity at 55°C and at 80°C
Pseudomonas sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.19
40
-
pH 6.8, in presence of Co2+, 10 min, stable up to
Pseudomonas sp.
3.5.1.19
70
-
10 min, complete loss of activity
Pseudomonas sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.19
7
9
-
Pseudomonas sp.
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.19
5
10.5
pH 5.0: about 70% of maximal activity, pH 10.5: about 75% of maximal activity
Pseudomonas sp.
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.1.19
enzyme is stable in buffer solution containing Co2+ and Ni2+
Pseudomonas sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.19
Cu2+
-
Pseudomonas sp.
3.5.1.19
Hg2+
-
Pseudomonas sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.005
-
nicotinamide
-
Pseudomonas sp.
3.5.1.19
0.056
-
Pyrazinamide
-
Pseudomonas sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.19
Co2+
enhances activity
Pseudomonas sp.
3.5.1.19
Mn2+
enhances activity
Pseudomonas sp.
3.5.1.19
Ni2+
enhances activity
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.19
26000
-
2 * 26000, SDS-PAGE
Pseudomonas sp.
3.5.1.19
43000
-
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.19
nicotinamide + H2O
Pseudomonas sp.
-
nicotinate + NH3
-
-
?
3.5.1.19
nicotinamide + H2O
Pseudomonas sp. GDI 211
-
nicotinate + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.19
-
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.19
37.6
-
-
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
246588
Pseudomonas sp.
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
59.5% of the activity with pyrazinamide
246588
Pseudomonas sp.
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
-
246588
Pseudomonas sp. GDI 211
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
59.5% of the activity with pyrazinamide
246588
Pseudomonas sp. GDI 211
nicotinate + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
246588
Pseudomonas sp.
pyrazinoic acid + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
246588
Pseudomonas sp. GDI 211
pyrazinoic acid + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.19
dimer
2 * 26000, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.19
75
-
-
Pseudomonas sp.
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.1.19
55
80
about 35% of maximal activity at 55°C and at 80°C
Pseudomonas sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.19
40
-
pH 6.8, in presence of Co2+, 10 min, stable up to
Pseudomonas sp.
3.5.1.19
70
-
10 min, complete loss of activity
Pseudomonas sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.19
7
9
-
Pseudomonas sp.
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.19
5
10.5
pH 5.0: about 70% of maximal activity, pH 10.5: about 75% of maximal activity
Pseudomonas sp.