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Literature summary extracted from

  • Kgayama, T.; Ohe, T.
    Purification and properties of an aromatic amidase from Pseudomonas sp. GDI 211 (1990), Agric. Biol. Chem., 54, 2565-2571.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.5.1.19 enzyme is stable in buffer solution containing Co2+ and Ni2+ Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.19 Cu2+
-
 Pseudomonas sp.
3.5.1.19 Hg2+
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.19 0.005
-
 nicotinamide
-
 Pseudomonas sp.
3.5.1.19 0.056
-
 Pyrazinamide
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.19 Co2+ enhances activity Pseudomonas sp.
3.5.1.19 Mn2+ enhances activity Pseudomonas sp.
3.5.1.19 Ni2+ enhances activity Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.19 26000
-
 2 * 26000, SDS-PAGE Pseudomonas sp.
3.5.1.19 43000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.19 nicotinamide + H2O Pseudomonas sp.
-
 nicotinate + NH3
-
 ?
3.5.1.19 nicotinamide + H2O Pseudomonas sp. GDI 211
-
 nicotinate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.19 Pseudomonas sp.
-
-
-
3.5.1.19 Pseudomonas sp. GDI 211
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.19
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.19 37.6
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.19 nicotinamide + H2O
-
 Pseudomonas sp. nicotinate + NH3
-
 ?
3.5.1.19 nicotinamide + H2O 59.5% of the activity with pyrazinamide Pseudomonas sp. nicotinate + NH3
-
 ?
3.5.1.19 nicotinamide + H2O
-
 Pseudomonas sp. GDI 211 nicotinate + NH3
-
 ?
3.5.1.19 nicotinamide + H2O 59.5% of the activity with pyrazinamide Pseudomonas sp. GDI 211 nicotinate + NH3
-
 ?
3.5.1.19 pyrazinamide + H2O
-
 Pseudomonas sp. pyrazinoic acid + NH3
-
 ?
3.5.1.19 pyrazinamide + H2O
-
 Pseudomonas sp. GDI 211 pyrazinoic acid + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.19 dimer 2 * 26000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.19 75
-
-
 Pseudomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.19 55 80 about 35% of maximal activity at 55°C and at 80°C Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.19 40
-
 pH 6.8, in presence of Co2+, 10 min, stable up to Pseudomonas sp.
3.5.1.19 70
-
 10 min, complete loss of activity Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.19 7 9
-
 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.19 5 10.5 pH 5.0: about 70% of maximal activity, pH 10.5: about 75% of maximal activity Pseudomonas sp.