BRENDA - Enzyme Database show

Purification and characterization of nicotinamide deamidase from yeast

Yan, C.; Sloan, D.L.; J. Biol. Chem. 262, 9082-9087 (1987)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.19
N-ethylmaleimide
-
Saccharomyces cerevisiae
3.5.1.19
nicotinaldehyde
-
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.033
-
nicotinamide
-
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.19
30000
-
gel filtration
Saccharomyces cerevisiae
3.5.1.19
34000
-
1 * 34000, SDS-PAGE
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.19
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.19
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.19
additional information
-
-
Saccharomyces cerevisiae
3.5.1.19
0.175
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
246581
Saccharomyces cerevisiae
nicotinate + NH3
-
-
-
ir
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.19
monomer
1 * 34000, SDS-PAGE
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.19
N-ethylmaleimide
-
Saccharomyces cerevisiae
3.5.1.19
nicotinaldehyde
-
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.033
-
nicotinamide
-
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.19
30000
-
gel filtration
Saccharomyces cerevisiae
3.5.1.19
34000
-
1 * 34000, SDS-PAGE
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.19
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.19
additional information
-
-
Saccharomyces cerevisiae
3.5.1.19
0.175
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
246581
Saccharomyces cerevisiae
nicotinate + NH3
-
-
-
ir
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.19
monomer
1 * 34000, SDS-PAGE
Saccharomyces cerevisiae