BRENDA - Enzyme Database show

Nicotinamide deamidase from Flavobacterium peregrinum

Tanigawa, Y.; Shimiyama, M.; Ueda, I.; Methods Enzymol. 66, 132-136 (1980)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.5.1.19
inactivated by dialysis in absence of Mn2+. After overnight dialysis in presence of Mn2+ almost all activity is retained. A dialysis against 0.01 M maleate buffer, pH 6.5, containing 0.005 mM HgCl2 results in complete retention of activity
Flavobacterium peregrinum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.19
3-acetylpyridine
competitive
Flavobacterium peregrinum
3.5.1.19
Cu2+
CuSO4
Flavobacterium peregrinum
3.5.1.19
EDTA
-
Flavobacterium peregrinum
3.5.1.19
Fe2+
FeCl2
Flavobacterium peregrinum
3.5.1.19
HgCl2
-
Flavobacterium peregrinum
3.5.1.19
N,N'-diethylnicotinamide
competitive
Flavobacterium peregrinum
3.5.1.19
N2-ethylnicotinamide
competitive
Flavobacterium peregrinum
3.5.1.19
N2-methylnicotinamide
competitive
Flavobacterium peregrinum
3.5.1.19
nicotinic acid hydrazide
competitive
Flavobacterium peregrinum
3.5.1.19
PCMB
-
Flavobacterium peregrinum
3.5.1.19
Zn2+
ZnCl2
Flavobacterium peregrinum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.0002
-
nicotinamide
-
Flavobacterium peregrinum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.19
48000
-
gel filtration
Flavobacterium peregrinum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.19
Flavobacterium peregrinum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.19
-
Flavobacterium peregrinum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.19
2.17
-
-
Flavobacterium peregrinum
Storage Stability
EC Number
Storage Stability
Organism
3.5.1.19
-20°C, 50% loss of activity after 4 weeks
Flavobacterium peregrinum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
246578
Flavobacterium peregrinum
nicotinate + NH3
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.19
6.5
-
-
Flavobacterium peregrinum
3.5.1.19
6.6
-
-
Flavobacterium peregrinum
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.1.19
inactivated by dialysis in absence of Mn2+. After overnight dialysis in presence of Mn2+ almost all activity is retained. A dialysis against 0.01 M maleate buffer, pH 6.5, containing 0.005 mM HgCl2 results in complete retention of activity
Flavobacterium peregrinum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.19
3-acetylpyridine
competitive
Flavobacterium peregrinum
3.5.1.19
Cu2+
CuSO4
Flavobacterium peregrinum
3.5.1.19
EDTA
-
Flavobacterium peregrinum
3.5.1.19
Fe2+
FeCl2
Flavobacterium peregrinum
3.5.1.19
HgCl2
-
Flavobacterium peregrinum
3.5.1.19
N,N'-diethylnicotinamide
competitive
Flavobacterium peregrinum
3.5.1.19
N2-ethylnicotinamide
competitive
Flavobacterium peregrinum
3.5.1.19
N2-methylnicotinamide
competitive
Flavobacterium peregrinum
3.5.1.19
nicotinic acid hydrazide
competitive
Flavobacterium peregrinum
3.5.1.19
PCMB
-
Flavobacterium peregrinum
3.5.1.19
Zn2+
ZnCl2
Flavobacterium peregrinum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.0002
-
nicotinamide
-
Flavobacterium peregrinum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.19
48000
-
gel filtration
Flavobacterium peregrinum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.19
-
Flavobacterium peregrinum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.19
2.17
-
-
Flavobacterium peregrinum
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.1.19
-20°C, 50% loss of activity after 4 weeks
Flavobacterium peregrinum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
246578
Flavobacterium peregrinum
nicotinate + NH3
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.19
6.5
-
-
Flavobacterium peregrinum
3.5.1.19
6.6
-
-
Flavobacterium peregrinum