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Purification and characterization of a nicotinamide deamidase released into the growth medium of neuroblastoma in vitro

Wintzerith, M.; Dierich, A.; Mandel, P.; Biochim. Biophys. Acta 613, 191-202 (1980)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.1.19
Serum albumin
0.02%, 9% activation
Mus musculus
General Stability
EC Number
General Stability
Organism
3.5.1.19
freezing and defreezing inactivates
Mus musculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.19
2-mercaptoethanol
-
Mus musculus
3.5.1.19
Ag+
-
Mus musculus
3.5.1.19
Cu2+
-
Mus musculus
3.5.1.19
Cys
-
Mus musculus
3.5.1.19
dithiothreitol
-
Mus musculus
3.5.1.19
EDTA
0.02 mM, 46% inhibition
Mus musculus
3.5.1.19
Fe2+
-
Mus musculus
3.5.1.19
Hg2+
-
Mus musculus
3.5.1.19
NAD+
competitive with respect to nicotinamide
Mus musculus
3.5.1.19
sulfhydryl reagents
-
Mus musculus
3.5.1.19
Triton X-100
0.1%, 24% inhibition
Mus musculus
3.5.1.19
Urea
1 M, 20% inhibition
Mus musculus
3.5.1.19
Zn2+
-
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
additional information
-
additional information
-
Mus musculus
3.5.1.19
0.006
-
nicotinamide
-
Mus musculus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.19
Ca2+
activation
Mus musculus
3.5.1.19
Mg2+
activation
Mus musculus
3.5.1.19
Mn2+
activation
Mus musculus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.19
50000
-
2 * 65000 + 2 * 50000, SDS-PAGE
Mus musculus
3.5.1.19
65000
-
2 * 65000 + 2 * 50000, SDS-PAGE
Mus musculus
3.5.1.19
230000
-
gel electrophoresis
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.19
nicotinamide + H2O
Mus musculus
enzyme is induced at high nicotinamide concentrations
nicotinate + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.19
Mus musculus
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.5.1.19
glycoprotein
contains 1.7% w/w mannose and 1.5% w/w N-acetylglucosamine
Mus musculus
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.19
-
Mus musculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.19
C-1300 cell
C1300 neuroblastoma M1 clonal cell line
Mus musculus
-
Storage Stability
EC Number
Storage Stability
Organism
3.5.1.19
-20°C, 50% glycerol, stable for months
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
246576
Mus musculus
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
enzyme is induced at high nicotinamide concentrations
246576
Mus musculus
nicotinate + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.19
tetramer
2 * 65000 + 2 * 50000, SDS-PAGE
Mus musculus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.19
37
-
-
Mus musculus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.19
55
-
half-life: 2.5 min
Mus musculus
3.5.1.19
65
-
half-life: 1 min
Mus musculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.19
6.8
7.5
-
Mus musculus
3.5.1.19
7.2
-
-
Mus musculus
3.5.1.19
7.5
-
-
Mus musculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.1.19
Serum albumin
0.02%, 9% activation
Mus musculus
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.1.19
freezing and defreezing inactivates
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.19
2-mercaptoethanol
-
Mus musculus
3.5.1.19
Ag+
-
Mus musculus
3.5.1.19
Cu2+
-
Mus musculus
3.5.1.19
Cys
-
Mus musculus
3.5.1.19
dithiothreitol
-
Mus musculus
3.5.1.19
EDTA
0.02 mM, 46% inhibition
Mus musculus
3.5.1.19
Fe2+
-
Mus musculus
3.5.1.19
Hg2+
-
Mus musculus
3.5.1.19
NAD+
competitive with respect to nicotinamide
Mus musculus
3.5.1.19
sulfhydryl reagents
-
Mus musculus
3.5.1.19
Triton X-100
0.1%, 24% inhibition
Mus musculus
3.5.1.19
Urea
1 M, 20% inhibition
Mus musculus
3.5.1.19
Zn2+
-
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
additional information
-
additional information
-
Mus musculus
3.5.1.19
0.006
-
nicotinamide
-
Mus musculus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.19
Ca2+
activation
Mus musculus
3.5.1.19
Mg2+
activation
Mus musculus
3.5.1.19
Mn2+
activation
Mus musculus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.19
50000
-
2 * 65000 + 2 * 50000, SDS-PAGE
Mus musculus
3.5.1.19
65000
-
2 * 65000 + 2 * 50000, SDS-PAGE
Mus musculus
3.5.1.19
230000
-
gel electrophoresis
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.19
nicotinamide + H2O
Mus musculus
enzyme is induced at high nicotinamide concentrations
nicotinate + NH3
-
-
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.5.1.19
glycoprotein
contains 1.7% w/w mannose and 1.5% w/w N-acetylglucosamine
Mus musculus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.19
-
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.19
C-1300 cell
C1300 neuroblastoma M1 clonal cell line
Mus musculus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.1.19
-20°C, 50% glycerol, stable for months
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
246576
Mus musculus
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
enzyme is induced at high nicotinamide concentrations
246576
Mus musculus
nicotinate + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.19
tetramer
2 * 65000 + 2 * 50000, SDS-PAGE
Mus musculus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.19
37
-
-
Mus musculus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.19
55
-
half-life: 2.5 min
Mus musculus
3.5.1.19
65
-
half-life: 1 min
Mus musculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.19
6.8
7.5
-
Mus musculus
3.5.1.19
7.2
-
-
Mus musculus
3.5.1.19
7.5
-
-
Mus musculus