BRENDA - Enzyme Database show

Kinetic studies of nicotinamide deaminase from Micrococcus lysodeikticus

Gadd, R.E.A.; Johson, W.J.; Int. J. Biochem. 5, 397-407 (1974)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.19
5-Methylnicotinamide
competitive
Micrococcus luteus
3.5.1.19
EDTA
2 mM, no effect
Micrococcus luteus
3.5.1.19
PCMB
-
Micrococcus luteus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.0029
-
nicotinamide
-
Micrococcus luteus
3.5.1.19
0.36
-
5'-methylnicotinamide
-
Micrococcus luteus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.19
Micrococcus luteus
-
i.e. Micrococcus lysodeiktikus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
5-methylnicotinamide + H2O
-
246573
Micrococcus luteus
5-methylnicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
-
246573
Micrococcus luteus
nicotinate + NH3
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.19
37
-
stable for more than 3 h
Micrococcus luteus
3.5.1.19
50
-
10 min, 27% loss of activity
Micrococcus luteus
3.5.1.19
60
-
10 min, 76% loss of activity
Micrococcus luteus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.1.19
7
9.5
37°C, 10 min, stable
Micrococcus luteus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.19
5-Methylnicotinamide
competitive
Micrococcus luteus
3.5.1.19
EDTA
2 mM, no effect
Micrococcus luteus
3.5.1.19
PCMB
-
Micrococcus luteus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.0029
-
nicotinamide
-
Micrococcus luteus
3.5.1.19
0.36
-
5'-methylnicotinamide
-
Micrococcus luteus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
5-methylnicotinamide + H2O
-
246573
Micrococcus luteus
5-methylnicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
-
246573
Micrococcus luteus
nicotinate + NH3
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.19
37
-
stable for more than 3 h
Micrococcus luteus
3.5.1.19
50
-
10 min, 27% loss of activity
Micrococcus luteus
3.5.1.19
60
-
10 min, 76% loss of activity
Micrococcus luteus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.1.19
7
9.5
37°C, 10 min, stable
Micrococcus luteus