BRENDA - Enzyme Database show

Purification and properties of nicotinamide deamidase from Flavobacterium peregrinum

Tanigawa, J.; Shimiyama, M.; Dohi, K.; Ueda, I.; J. Biol. Chem. 247, 8036-8042 (1972)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.1.19
EDTA
less than 0.5 mM, slight enhancement of deaminase activity
Flavobacterium peregrinum
General Stability
EC Number
General Stability
Organism
3.5.1.19
inactivated by dialysis in absence of Mn2+. After overnight dialysis in presence of Mn2+ almost all activity is retained. A dialysis against 0.01 M maleate buffer, pH 6.5, containing 0.005 mM HgCl2 results in complete retention of activity
Flavobacterium peregrinum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.19
Cu2+
CuSO4
Flavobacterium peregrinum
3.5.1.19
Fe2+
-
Flavobacterium peregrinum
3.5.1.19
HgCl2
-
Flavobacterium peregrinum
3.5.1.19
nicotinamide analogs
analogs with a trivalent nitrogen, competitive inhibition
Flavobacterium peregrinum
3.5.1.19
PCMB
-
Flavobacterium peregrinum
3.5.1.19
Zn2+
ZnCl2
Flavobacterium peregrinum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.0002
-
nicotinamide
-
Flavobacterium peregrinum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.19
48000
-
gel filtration
Flavobacterium peregrinum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.19
Flavobacterium peregrinum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.19
-
Flavobacterium peregrinum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.19
additional information
-
-
Flavobacterium peregrinum
3.5.1.19
2.17
-
-
Flavobacterium peregrinum
Storage Stability
EC Number
Storage Stability
Organism
3.5.1.19
-20°C, 50% loss of activity after 4 weeks
Flavobacterium peregrinum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
246571
Flavobacterium peregrinum
nicotinate + NH3
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.19
6.5
-
-
Flavobacterium peregrinum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.1.19
EDTA
less than 0.5 mM, slight enhancement of deaminase activity
Flavobacterium peregrinum
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.1.19
inactivated by dialysis in absence of Mn2+. After overnight dialysis in presence of Mn2+ almost all activity is retained. A dialysis against 0.01 M maleate buffer, pH 6.5, containing 0.005 mM HgCl2 results in complete retention of activity
Flavobacterium peregrinum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.19
Cu2+
CuSO4
Flavobacterium peregrinum
3.5.1.19
Fe2+
-
Flavobacterium peregrinum
3.5.1.19
HgCl2
-
Flavobacterium peregrinum
3.5.1.19
nicotinamide analogs
analogs with a trivalent nitrogen, competitive inhibition
Flavobacterium peregrinum
3.5.1.19
PCMB
-
Flavobacterium peregrinum
3.5.1.19
Zn2+
ZnCl2
Flavobacterium peregrinum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.0002
-
nicotinamide
-
Flavobacterium peregrinum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.19
48000
-
gel filtration
Flavobacterium peregrinum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.19
-
Flavobacterium peregrinum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.19
additional information
-
-
Flavobacterium peregrinum
3.5.1.19
2.17
-
-
Flavobacterium peregrinum
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.1.19
-20°C, 50% loss of activity after 4 weeks
Flavobacterium peregrinum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
246571
Flavobacterium peregrinum
nicotinate + NH3
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.19
6.5
-
-
Flavobacterium peregrinum