BRENDA - Enzyme Database show

Active-site studies on rabbit liver nicotinamide deamidase

Albizati, L.D.; Hedrick, J.L.; Biochemistry 11, 1508-1516 (1972)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.19
3-indolepropionic acid
inhibits amidase and esterase activity
Oryctolagus cuniculus
3.5.1.19
Benzoic acid
inhibits amidase and esterase activity
Oryctolagus cuniculus
3.5.1.19
diisopropylfluorophosphate
-
Oryctolagus cuniculus
3.5.1.19
Hydrocinnamic acid
inhibits amidase and esterase activity
Oryctolagus cuniculus
3.5.1.19
nicotinamide
inhibits esterolysis of p-nitrophenyl acetate
Oryctolagus cuniculus
3.5.1.19
nicotinic acid
inhibits amidase and esterase activity
Oryctolagus cuniculus
3.5.1.19
p-nitrophenol
inhibits amidase and esterase activity
Oryctolagus cuniculus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.19
60000
-
4 * 60000, rabbit, SDS-PAGE
Oryctolagus cuniculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.19
Oryctolagus cuniculus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.19
liver
-
Oryctolagus cuniculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
246570
Oryctolagus cuniculus
nicotinate + NH3
-
-
-
?
3.5.1.19
p-nitrophenol + H2O
-
246570
Oryctolagus cuniculus
phenol + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.19
tetramer
4 * 60000, rabbit, SDS-PAGE
Oryctolagus cuniculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.19
6.5
8.5
amidase activity
Oryctolagus cuniculus
3.5.1.19
8
10
esterase
Oryctolagus cuniculus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.19
5.5
9.5
pH 5.5: about 60% of maximal activity, pH 9.5: about 45% of maximal activity
Oryctolagus cuniculus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.1.19
5
10
37°C, 1 h, stable
Oryctolagus cuniculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.19
3-indolepropionic acid
inhibits amidase and esterase activity
Oryctolagus cuniculus
3.5.1.19
Benzoic acid
inhibits amidase and esterase activity
Oryctolagus cuniculus
3.5.1.19
diisopropylfluorophosphate
-
Oryctolagus cuniculus
3.5.1.19
Hydrocinnamic acid
inhibits amidase and esterase activity
Oryctolagus cuniculus
3.5.1.19
nicotinamide
inhibits esterolysis of p-nitrophenyl acetate
Oryctolagus cuniculus
3.5.1.19
nicotinic acid
inhibits amidase and esterase activity
Oryctolagus cuniculus
3.5.1.19
p-nitrophenol
inhibits amidase and esterase activity
Oryctolagus cuniculus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.19
60000
-
4 * 60000, rabbit, SDS-PAGE
Oryctolagus cuniculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.19
liver
-
Oryctolagus cuniculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
246570
Oryctolagus cuniculus
nicotinate + NH3
-
-
-
?
3.5.1.19
p-nitrophenol + H2O
-
246570
Oryctolagus cuniculus
phenol + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.19
tetramer
4 * 60000, rabbit, SDS-PAGE
Oryctolagus cuniculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.19
6.5
8.5
amidase activity
Oryctolagus cuniculus
3.5.1.19
8
10
esterase
Oryctolagus cuniculus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.19
5.5
9.5
pH 5.5: about 60% of maximal activity, pH 9.5: about 45% of maximal activity
Oryctolagus cuniculus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.1.19
5
10
37°C, 1 h, stable
Oryctolagus cuniculus