BRENDA - Enzyme Database show

Hyperproduction and purification of nicotinamide deamidase, a microconstitutive enzyme of Escherichia coli

Pardee, B.P.; J. Biol. Chem. 246, 6792-6796 (1971)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.07
-
nicotinamide
-
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.19
30000
-
gel filtration
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.19
Escherichia coli
-
K12
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.19
-
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.19
27.2
-
-
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
3.5.1.19
0°C, phosphate buffer, pH 7.0, weak loss of activity
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
246569
Escherichia coli
nicotinate + NH3
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.19
37
-
half-life: 2 h, stable in presence of 0.05 mM nicotinamide
Escherichia coli
3.5.1.19
50
-
5 min, 50% loss of activity
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.19
7.2
-
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.07
-
nicotinamide
-
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.19
30000
-
gel filtration
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.19
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.19
27.2
-
-
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.1.19
0°C, phosphate buffer, pH 7.0, weak loss of activity
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
246569
Escherichia coli
nicotinate + NH3
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.19
37
-
half-life: 2 h, stable in presence of 0.05 mM nicotinamide
Escherichia coli
3.5.1.19
50
-
5 min, 50% loss of activity
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.19
7.2
-
-
Escherichia coli