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Literature summary extracted from
- Hydrolysis of N-succinyl-L,L-diaminopimelic acid by Haemophilus influenzae dapE-encoded desuccinylase: metal activation, solvent isotope effects, and kinetic mechanism (1998), Biochemistry, 37, 10478-10487.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.18 | expression in Escherichia coli | Haemophilus influenzae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.18 | D,L-diaminopimelic acid | competitive | Haemophilus influenzae |  |
| 3.5.1.18 | EDTA | complete | Haemophilus influenzae | |
| 3.5.1.18 | L,L-diaminopimelic acid | competitive | Haemophilus influenzae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.18 | 1.3 | - |
N-succinyl-LL-2,6-diaminoheptanedioate | in the presence of Zn2+ | Haemophilus influenzae | |
| 3.5.1.18 | 1.6 | - |
N-succinyl-LL-2,6-diaminoheptanedioate | in the presence of Co2+ | Haemophilus influenzae | |
| 3.5.1.18 | 3.2 | - |
N-succinyl-DL-2,6-diaminoheptanedioate | in the presence of Zn2+ | Haemophilus influenzae | |
| 3.5.1.18 | 4.7 | - |
N-succinyl-LL-2,6-diaminoheptanedioate | in the presence of Co2+ | Haemophilus influenzae | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.18 | Co2+ | - |
Haemophilus influenzae | |
| 3.5.1.18 | Zn2+ | required | Haemophilus influenzae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.18 | 41350 | - |
1 * 41350, electrospray mass spectroscopy | Haemophilus influenzae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.18 | additional information | Haemophilus influenzae | kinetic mechanism | ? | - |
? | |
| 3.5.1.18 | additional information | Haemophilus influenzae RD | kinetic mechanism | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.18 | Haemophilus influenzae | - |
RD | - |
| 3.5.1.18 | Haemophilus influenzae RD | - |
RD | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.18 | - |
Haemophilus influenzae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.18 | additional information | kinetic mechanism | Haemophilus influenzae | ? | - |
? | |
| 3.5.1.18 | additional information | kinetic mechanism | Haemophilus influenzae RD | ? | - |
? | |
| 3.5.1.18 | N-succinyl-DL-2,6-diaminoheptanedioate + H2O | - |
Haemophilus influenzae | succinate + LL-2,6-diaminoheptanedioate | - |
? | |
| 3.5.1.18 | N-succinyl-DL-2,6-diaminoheptanedioate + H2O | - |
Haemophilus influenzae RD | succinate + LL-2,6-diaminoheptanedioate | - |
? | |
| 3.5.1.18 | N-succinyl-LL-2,6-diaminoheptanedioate + H2O | - |
Haemophilus influenzae | succinate + LL-2,6-diaminoheptanedioate | - |
? | |
| 3.5.1.18 | N-succinyl-LL-2,6-diaminoheptanedioate + H2O | - |
Haemophilus influenzae RD | succinate + LL-2,6-diaminoheptanedioate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.18 | monomer | 1 * 41350, electrospray mass spectroscopy | Haemophilus influenzae |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.18 | 0.0555 | - |
N-succinyl-LL-2,6-diaminoheptanedioate | in the presence of zinc | Haemophilus influenzae | |
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