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Literature summary extracted from

  • Munford, R.S.; Hall, C.L.
    Purification of acyloxyacyl hydrolase, a leukocyte enzyme that removes secondary acyl chains from bacterial lipopolysaccharides (1989), J. Biol. Chem., 264, 15613-15619.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.77 dimethylsulfate induces enzyme activity when added to the medium Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.77 2-mercaptoethanol complete inhibition at 0.1 M Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.77 0.00055
-
lipopolysaccharide
-
Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.77 membrane
-
Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.77 14000 20000 small subunit, SDS-PAGE in presence of 2-mercaptoethanol Homo sapiens
3.1.1.77 48000
-
SDS-PAGE of large subunit after N-glycanase treatment Homo sapiens
3.1.1.77 50000
-
large subunit, SDS-PAGE in presence of 2-mercaptoethanol Homo sapiens
3.1.1.77 52000 60000 native PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.77 3-(acyloxy)acyl group of bacterial toxin Homo sapiens detoxification of LPS 3-hydroxyacyl group of bacterial toxin + a fatty acid
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.77 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.77 glycoprotein both subunits glycosylated Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.77
-
Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.77 HL-60 cell
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.77 0.000005
-
cell lysate Homo sapiens
3.1.1.77 1.555
-
purified enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.77 3-(acyloxy)acyl group of bacterial toxin detoxification of LPS Homo sapiens 3-hydroxyacyl group of bacterial toxin + a fatty acid
-
?
3.1.1.77 3-(acyloxy)acyl group of bacterial toxin + H2O
-
Homo sapiens 3-hydroxyacyl group of bacterial toxin + a fatty acid
-
?
3.1.1.77 lipopolysaccharide + H2O
-
Homo sapiens ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.77 dimer alpha, beta, 1 * 50000 + 1 * 14000-20000, disulfide linked Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.77 4.8 5
-
Homo sapiens