Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jไger, K.E.; Steinbuechel, A.; Jendrossek, D.
    Substrate specificities of bacterial polyhydroxyalkanoate depolymerases and lipases: bacterial lipases hydolyze poly(omega-hydroxyalkanoates) (1995), Appl. Environ. Microbiol., 61, 3113-3118.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular
-
 Bacillus subtilis
-
-
3.1.1.3 extracellular
-
 Pseudomonas fluorescens
-
-
3.1.1.3 extracellular
-
 Pseudomonas aeruginosa
-
-
3.1.1.3 extracellular
-
 Pseudomonas alcaligenes
-
-
3.1.1.3 extracellular
-
 Burkholderia cepacia
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 19400
-
 x * 19400, SDS-PAGE Bacillus subtilis
3.1.1.3 29000
-
 x * 29000, SDS-PAGE Pseudomonas aeruginosa
3.1.1.3 30000
-
 x * 30000, SDS-PAGE Pseudomonas alcaligenes
3.1.1.3 33000
-
 x * 33000, SDS-PAGE Burkholderia cepacia
3.1.1.3 48000
-
 x * 48000, recombinant enzyme, SDS-PAGE Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.76 poly[(R)-3-hydroxyoctanoate]n + H2O Pseudomonas fluorescens utilization of bacterial PHA storage compounds poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x x = 1-5 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Bacillus subtilis
-
-
-
3.1.1.3 Burkholderia cepacia
-
-
-
3.1.1.3 Pseudomonas aeruginosa
-
-
-
3.1.1.3 Pseudomonas alcaligenes
-
-
-
3.1.1.3 Pseudomonas fluorescens
-
-
-
3.1.1.76 Pseudomonas fluorescens
-
 GK13
-
3.1.1.76 Pseudomonas fluorescens GK13
-
 GK13
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3
-
 Bacillus subtilis
3.1.1.3
-
 Pseudomonas aeruginosa
3.1.1.3
-
 Pseudomonas alcaligenes
3.1.1.3
-
 Burkholderia cepacia
3.1.1.3 recombinant enzyme Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 1,2-dilauryl-rac-glycero-3-glutaric acid resorufinester + H2O
-
 Bacillus subtilis ?
-
 ?
3.1.1.3 1,2-dilauryl-rac-glycero-3-glutaric acid resorufinester + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.1.1.3 1,2-dilauryl-rac-glycero-3-glutaric acid resorufinester + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.1.1.3 1,2-dilauryl-rac-glycero-3-glutaric acid resorufinester + H2O
-
 Pseudomonas alcaligenes ?
-
 ?
3.1.1.3 1,2-dilauryl-rac-glycero-3-glutaric acid resorufinester + H2O
-
 Burkholderia cepacia ?
-
 ?
3.1.1.3 1,2-dilauryl-rac-glycero-3-glutaric acid resorufinester + H2O
-
 Pseudomonas fluorescens GK13 ?
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Bacillus subtilis 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Pseudomonas fluorescens 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Pseudomonas aeruginosa 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Pseudomonas alcaligenes 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Burkholderia cepacia 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Pseudomonas fluorescens GK13 4-nitrophenol + palmitate
-
 ?
3.1.1.3 additional information substrate specificity of the extracellular enzyme Pseudomonas alcaligenes ?
-
 ?
3.1.1.3 additional information substrate specificity of the extracellular enzyme Burkholderia cepacia ?
-
 ?
3.1.1.3 additional information substrate specificity of the extracellular enzyme, the enzyme shows high activity Pseudomonas aeruginosa ?
-
 ?
3.1.1.3 additional information substrate specificity of the extracellular enzyme, the enzyme shows low activity Bacillus subtilis ?
-
 ?
3.1.1.3 additional information substrate specificity of the recombinant extracellular enzyme overexpressed in Escherichia coli Pseudomonas fluorescens ?
-
 ?
3.1.1.3 additional information substrate specificity of the recombinant extracellular enzyme overexpressed in Escherichia coli Pseudomonas fluorescens GK13 ?
-
 ?
3.1.1.3 triolein + H2O
-
 Bacillus subtilis diolein + oleate
-
 ?
3.1.1.3 triolein + H2O
-
 Pseudomonas fluorescens diolein + oleate
-
 ?
3.1.1.3 triolein + H2O
-
 Pseudomonas aeruginosa diolein + oleate
-
 ?
3.1.1.3 triolein + H2O
-
 Pseudomonas alcaligenes diolein + oleate
-
 ?
3.1.1.3 triolein + H2O
-
 Burkholderia cepacia diolein + oleate
-
 ?
3.1.1.3 triolein + H2O
-
 Pseudomonas fluorescens GK13 diolein + oleate
-
 ?
3.1.1.76 poly[(R)-3-hydroxyoctanoate]n + H2O able to hydrolyze poly(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyalkanote) together with poly(3hydroxyoctanoate) Pseudomonas fluorescens poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x x: 1-5 ?
3.1.1.76 poly[(R)-3-hydroxyoctanoate]n + H2O utilization of bacterial PHA storage compounds Pseudomonas fluorescens poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x x = 1-5 ?
3.1.1.76 poly[(R)-3-hydroxyoctanoate]n + H2O able to hydrolyze poly(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyalkanote) together with poly(3hydroxyoctanoate) Pseudomonas fluorescens GK13 poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x x: 1-5 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 30000, SDS-PAGE Pseudomonas alcaligenes
3.1.1.3 ? x * 29000, SDS-PAGE Pseudomonas aeruginosa
3.1.1.3 ? x * 33000, SDS-PAGE Burkholderia cepacia
3.1.1.3 ? x * 19400, SDS-PAGE Bacillus subtilis
3.1.1.3 ? x * 48000, recombinant enzyme, SDS-PAGE Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Bacillus subtilis
3.1.1.3 lipase
-
 Pseudomonas fluorescens
3.1.1.3 lipase
-
 Pseudomonas aeruginosa
3.1.1.3 lipase
-
 Pseudomonas alcaligenes
3.1.1.3 lipase
-
 Burkholderia cepacia