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Literature summary extracted from

  • Vedadi, M.; Barriault, D.; Sylvestre, M; Powlowski, J.
    Active site residues of cis-2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase from Comamonas testosteroni strain B-356 (2000), Biochemistry, 39, 5028-5034.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.56 0.00024
-
 NAD(P)+ at pH 9 and 25°C Comamonas testosteroni
1.3.1.56 0.0031
-
 cis-2,3-dihydro-2,3-dihydroxybiphenyl at pH 9 and 25°C Comamonas testosteroni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.56 29400
-
 4 * 29400, electrospray mass spectrometry Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.56 cis-2,3-dihydro-2,3-dihydroxybiphenyl + NAD(P)+ Comamonas testosteroni second step of the metabolic pathway for the bacterial degradation of biphenyl 2,3-dihydroxybiphenyl + NAD(P)H
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.56 Comamonas testosteroni
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.56 ammonium sulfate precipitation, DEAE-Sepharose chromatography, yield about 50 mg of enzyme/l of cell culture Comamonas testosteroni

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.56 17.4
-
 after purification at 25°C and pH 9 Comamonas testosteroni

Storage Stability

EC Number Storage Stability Organism
1.3.1.56 -20°C stable in 50% glycerol for months Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.56 cis-2,3-dihydro-2,3-dihydroxybiphenyl + NAD(P)+
-
 Comamonas testosteroni 2,3-dihydroxybiphenyl + NAD(P)H
-
 ?
1.3.1.56 cis-2,3-dihydro-2,3-dihydroxybiphenyl + NAD(P)+ second step of the metabolic pathway for the bacterial degradation of biphenyl Comamonas testosteroni 2,3-dihydroxybiphenyl + NAD(P)H
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.56 tetramer 4 * 29400, electrospray mass spectrometry Comamonas testosteroni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.56 9 9.5
-
 Comamonas testosteroni

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.56 NAD(P)+
-
 Comamonas testosteroni